LOCUS p7N-35S-Ubi10-RN 11474 bp DNA circular SYN 12-JUL-2013 DEFINITION Ligation of inverted 670pD1-35s-Ubi10-RNAi* into p7N* ACCESSION p7N-35S-Ubi10-RN KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 11474) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: p7N* Fragment ends: SfiI Fragment size: 8727 Insert molecule: 670pD1-35s-Ubi10-RNAi* Fragment ends: SfiI Fragment size: 2747 FEATURES Location/Qualifiers misc_feature 7..304 /gene="Pnos" /product="Promotor" /SECDrawAs="Region" /SECStyleId=1 /SECName="Pnos" /SECDescr="Promotor" misc_feature 319..870 /gene="bar" /product="Basta-Resistenz" /SECDrawAs="Gene" /SECStyleId=1 /SECName="bar" /SECDescr="Basta-Resistenz" misc_feature 893..1093 /gene="T35S" /product="Terminator" /SECDrawAs="Region" /SECStyleId=1 /SECName="T35S" /SECDescr="Terminator" misc_feature 1152..1664 /gene="P35S" /product="Promotor" /SECDrawAs="Region" /SECStyleId=1 misc_feature complement(1678..1726) /gene="T35s" /SECDrawAs="Region" /SECStyleId=1 CDS 1746..2295 /SECDrawAs="Gene" /SECStyleId=1 CDS complement(1746..2295) /gene="Gus" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 2316..2364 /gene="T35S" /SECDrawAs="Region" /SECStyleId=1 misc_feature complement(2378..3853) /gene="Ubq10At" /product="promoter" /SECDrawAs="Region" /SECStyleId=1 misc_feature complement(2380..2683) /gene="íntron" /SECDrawAs="Region" /SECStyleId=1 misc_feature 3949..3972 /gene="RB" /product="Right Border" /SECDrawAs="Region" /SECStyleId=1 /SECName="RB" /SECDescr="Right Border" misc_feature 4507..5204 /gene="ColE1" /product="ColE1 ORI" /SECDrawAs="Region" /SECStyleId=1 /SECName="ColE1" /SECDescr="ColE1 ORI" misc_feature 5753..9458 /gene="pVS1 ORI" /product="pVS1 ORI" /SECDrawAs="Region" /SECStyleId=1 /SECName="pVS1 ORI" /SECDescr="pVS1 ORI" CDS 9642..10652 /gene="Sm/Sp" /product="Strep/Spec resistence" /SECDrawAs="Gene" /SECStyleId=1 /SECName="Sm/Sp" /SECDescr="Strep/Spec resistence" misc_feature 11173..11196 /gene="LB" /product="Left Border" /SECDrawAs="Region" /SECStyleId=1 /SECName="LB" /SECDescr="Left Border" ORIGIN 1 tctagatcat gagcggagaa ttaagggagt cacgttatga cccccgccga tgacgcggga 61 caagccgttt tacgtttgga actgacagaa ccgcaacgtt gaaggagcca ctcagccgcg 121 ggtttctgga gtttaatgag ctaagcacat acgtcagaaa ccattattgc gcgttcaaaa 181 gtcgcctaag gtcactatca gctagcaaat atttcttgtc aaaaatgctc cactgacgtt 241 ccataaattc ccctcggtat ccaattagag tctcatattc actctcaatc caaataatct 301 gcacctcgag tggccaccat gggcccagaa cgacgcccgg ccgacatccg ccgtgccacc 361 gaggcggaca tgccggcggt ctgcaccatc gtcaaccact acatcgagac aagcacggtc 421 aacttccgta ccgagccgca ggaaccgcag gagtggacgg acgacctcgt ccgtctgcgg 481 gagcgctatc cctggctcgt cgccgaggtg gacggcgagg tcgccggcat cgcctacgcg 541 ggcccctgga aggcacgcaa cgcctacgac tggacggccg agtcgaccgt gtacgtctcc 601 ccccgccacc agcggacggg actgggctcc acgctctaca cccacctgct gaagtccctg 661 gaggcacagg gcttcaagag cgtggtcgct gtcatcgggc tgcccaacga cccgagcgtg 721 cgcatgcacg aggcgctcgg atatgccccc cgcggcatgc tgcgggcggc cggcttcaag 781 cacgggaact ggcatgacgt gggtttctgg cagctggact tcagcctgcc ggtaccgccc 841 cgtccggtcc tgcccgtcac cgagatctga gatcacgcgt tctagtccgc aaaaatcacc 901 agtctctctc tacaaatcta tctctctcta tttttctcca gaataatgtg tgagtagttc 961 ccagataagg gaattagggt tcttataggg tttcgctcat gtgttgagca tataagaaac 1021 ccttagtatg tatttgtatt tgtaaaatac ttctatcaat aaaatttcta attcctaaaa 1081 ccaaaatcca gtgacctgca ggcatgcaag ctgatccact agaggccatg ggggccaatt 1141 taaatggtac caatagagga cctaacagaa ctcgccgtaa agactggcga acagttcata 1201 cagagtctct tacgactcaa tgacaagaag aaaatcttcg tcaacatggt ggagcacgac 1261 acacttgtct actccaaaaa tatcaaagat acagtctcag aagaccaaag ggcaattgag 1321 acttttcaac aaagggtaat atccggaaac ctcctcggat tccattgccc agctatctgt 1381 cactttattg tgaagatagt ggaaaaggaa ggtggctcct acaaatgcca tcattgcgat 1441 aaaggaaagg ccatcgttga agatgcctct gccgacagtg gtcccaaaga tggaccccca 1501 cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat 1561 tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc ttcgcaagac 1621 ccttcctcta tataaggaag ttcatttcat ttggagagaa cacggtcgac tgagctcgtt 1681 ttaggaatta gagattttat tgagagcagt attttacaaa tacaaacgcg tctgtacata 1741 agcttcaccc aggtgttcgg cgtggtgtag agcattacgc tgcgatggat tccggcatag 1801 ttaaagaaat catggaagta agactgcttt ttcttgccgt tttcgtcggt aatcaccatt 1861 cccggcggga tagtctgcca gttcagttcg ttgttcacac aaacggtgat acgtacactt 1921 ttcccggcaa taacatacgg cgtgacatcg gcttcaaatg gcgtatagcc gccctgatgc 1981 tccatcactt cctgattatt gacccacact ttgccgtaat gagtgaccgc atcgaaacgc 2041 agcacgatac gctggcctgc ccaacctttc ggtataaaga cttcgcgctg ataccagacg 2101 ttgcccgcat aattacgaat atctgcatcg gcgaactgat cgttaaaact gcctggcaca 2161 gcaattgccc ggctttcttg taacgcgctt tcccaccaac gctgatcaat tccacagttt 2221 tcgcgatcca gactgaatgc ccacaggccg tcgagttttt tgatttcacg ggttggggtt 2281 tctacaggac gtaaccttaa gacccgggag gatcctttgt atttgtattt atcagcacac 2341 aataaataca cattttccta aaacggcgcc aactagtacc tgttaatcag aaaaactcag 2401 attaatcgac aaattcgatc gcacaaacta gaaactaaca ccagatctag atagaaatca 2461 caaatcgaag agtaattatt cgacaaaact caaattattt gaacaaatcg gatgatatct 2521 atgaaaccct aatcgagaat taagatgata tctaacgatc aaacccagaa aatcgtcttc 2581 gatctaagat taacagaatc taaaccaaag aacatatacg aaattgggat cgaacgaaaa 2641 caaaatcgaa gattttgaga gaataaggaa cacagaaatt taccttgatc acggtagaga 2701 gaattgagag aaagttttta agattttgag aaattgaaat ctgaattgtg aagaagaaga 2761 gctctttggg tattgtttta tagaagaaga agaagaaaag acgaggacga ctaggtcacg 2821 agaaagctaa ggcggtgaag caatagctaa taataaaatg acacgtgtat tgagcgttgt 2881 ttacacgcaa agttgttttt ggctaattgc cttattttta ggttgaggaa aagtatttgt 2941 gctttgagtt gataaacacg actcgtgtgt gccggctgca accactttga cgccgtttat 3001 tactgactcg tcgacaacca caatttctaa cggtcgtcat aagatccagc cgttgagatt 3061 taacgatcgt tacgatttat atttttttag cattatcgtt ttatttttta aatatacggt 3121 ggagctgaaa attggcaata attgaaccgt gggtcccact gcattgaagc gtatttcgta 3181 ttttctagaa ttcttcgtgc tttatttctt ttcctttttg tttttttttg ccatttatct 3241 aatgcaagtg ggcttataaa atcagtgaat ttcttggaaa agtaacttct ttatcgtata 3301 acatattgtg aaattatcca tttcttttaa ttttttagtg ttattggata tttttgtatg 3361 attattgatt tgcataggat aatgactttt gtatcaagtt ggtgaacaag tctcgttaaa 3421 aaaggcaagt ggtttggtga ctcgatttat tcttgttatt taattcatat atcaatggat 3481 cttatttggg gcctggtcca tatttaacac tcgtgttcag tccaatgacc aataatattt 3541 tttcattaat aacaatgtaa caagaatgat acacaaaaca ttctttgaat aagttcgcta 3601 tgaagaaggg aacttatccg gtcctagatc atcagttcat acaaacctcc atagagttca 3661 acatcttaaa caagaatatc ctgatctgaa gaatgtggag gctttagtcc cttggatact 3721 tgggaggctg tggaagaaca gaaacgagct ggtgcttaaa gggagggaat ttggaaccaa 3781 tgaggtatta gtaaggacac aagaagatgc agatgagtgg attagaagga aagaggctca 3841 gaatgtaagg aaagcggccg cattaattaa ggccctaagg gccagatctt gggcccggta 3901 cccgatcaga ttgtcgtttc ccgccttcgg tttaaactat cagtgtttga caggatatat 3961 tggcgggtaa acctaagaga aaagagcgtt tattagaata atcggatatt taaaagggcg 4021 tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg 4081 gagtgcttgg cattccgtgc gataatgact tctgttcaac cacccaaacg tcggaaagcc 4141 tgacgacgga gcagcattcc aaaaagatcc cttggctcgt ctgggtcggc tagaaggtcg 4201 agtgggctgc tgtggcttga tccctcaacg cggtcgcgga cgtagcgcag cgccgaaaaa 4261 tcctcgatcg caaatccgac gctgtcgaaa agcgtgatct gcttgtcgct ctttcggccg 4321 acgtcctggc cagtcatcac gcgccaaagt tccgtcacag gatgatctgg cgcgagttgc 4381 tggatctcgc cttcaatccg ggtctgtggc gggaactcca cgaaaatatc cgaacgcagc 4441 aagatatcgc ggtgcatctc ggtcttgcct gggcagtcgc cgccgacgcc gttgatgtgg 4501 acgccgaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa 4561 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga 4621 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg 4681 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc 4741 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag 4801 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc 4861 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg 4921 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac 4981 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga 5041 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt 5101 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag 5161 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg 5221 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta 5281 tcccctgatt ctgtggataa ccgattaccg cctttgagtg agctgatacc gctcgccgca 5341 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt 5401 attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa 5461 tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt 5521 catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct 5581 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt 5641 ttcaccgtca tcaccgaaac gcgcgaggca ggggtacgtc gaggtcgatc caacccctcc 5701 gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc gtcttctgaa aacgacatgt 5761 cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc cttttcctgg cgttttcttg 5821 tcgcgtgttt tagtcgcata aagtagaata cttgcgacta gaaccggaga cattacgcca 5881 tgaacaagag cgccgccgct ggcctgctgg gctatgcccg cgtcagcacc gacgaccagg 5941 acttgaccaa ccaacgggcc gaactgcacg cggccggctg caccaagctg ttttccgaga 6001 agatcaccgg caccaggcgc gaccgcccgg agctggccag gatgcttgac cacctacgcc 6061 ctggcgacgt tgtgacagtg accaggctag accgcctggc ccgcagcacc cgcgacctac 6121 tggacattgc cgagcgcatc caggaggccg gcgcgggcct gcgtagcctg gcagagccgt 6181 gggccgacac caccacgccg gccggccgca tggtgttgac cgtgttcgcc ggcattgccg 6241 agttcgagcg ttccctaatc atcgaccgca cccggagcgg gcgcgaggcc gccaaggcgc 6301 gaggcgtgaa gtttggcccc cgccctaccc tcaccccggc acagatcgcg cacgcccgcg 6361 agctgatcga ccaggaaggc cgcaccgtga aagaggcggc tgcactgctt ggcgtgcatc 6421 gctcgaccct gtaccgcgca cttgagcgca gcgaggaagt gacgcccacc gaggccaggc 6481 ggcgcggtgc cttccgtgag gacgcattga ccgaggccga cgccctggcg gccgccgaga 6541 atgaacgcca agaggaacaa gcatgaaacc gcaccaggac ggccaggacg aaccgttttt 6601 cattaccgaa gagatcgagg cggagatgat cgcggccggg tacgtgttcg agccgcccgc 6661 gcacgtctca accgtgcggc tgcatgaaat cctggccggt ttgtctgatg ccaagctcgc 6721 ggcctggccg gcgagcttgg ccgctgaaga aaccgagcgc cgccgtctaa aaaggtgatg 6781 tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg tatatgatgc gatgagtaaa 6841 taaacaaata cgcaagggga acgcatgaag gttatcgctg tacttaacca gaaaggcggg 6901 tcaggcaaga cgaccatcgc aacccatcta gcccgcgccc tgcaactcgc cggggccgat 6961 gttctgttag tcgattccga tccccagggc agtgcccgcg attgggcggc cgtgcgggaa 7021 gatcaaccgc taaccgttgt cggcatcgac cgcccgacga ttgaccgcga cgtgaaggcc 7081 atcggccggc gcgacttcgt agtgatcgac ggagcgcccc aggcggcgga cttggctgtg 7141 tccgcgatca aggcagccga cttcgtgctg attccggtgc agccaagccc ttacgacata 7201 tgggccaccg ccgacctggt ggagctggtt aagcagcgca ttgaggtcac ggatggaagg 7261 ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca cgcgcatcgg cggtgaggtt 7321 gccgaggcgc tggccgggta cgagctgccc attcttgagt cccgtatcac gcagcgcgtg 7381 agctacccag gcactgccgc cgccggcaca accgttcttg aatcagaacc cgagggcgac 7441 gctgcccgcg aggtccaggc gctggccgct gaaattaaat caaaactcat ttgagttaat 7501 gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag tgccggccgt ccgagcgcac 7561 gcagcagcaa ggctgcaacg ttggccagcc tggcagacac gccagccatg aagcgggtca 7621 actttcagtt gccggcggag gatcacacca agctgaagat gtacgcggta cgccaaggca 7681 agaccattac cgagctgcta tctgaataca tcgcgcagct accagagtaa atgagcaaat 7741 gaataaatga gtagatgaat tttagcggct aaaggaggcg gcatggaaaa tcaagaacaa 7801 ccaggcaccg acgccgtgga atgccccatg tgtggaggaa cgggcggttg gccaggcgta 7861 agcggctggg ttgtctgccg gccctgcaat ggcactggaa cccccaagcc cgaggaatcg 7921 gcgtgagcgg tcgcaaacca tccggcccgg tacaaatcgg cgcggcgctg ggtgatgacc 7981 tggtggagaa gttgaaggcg gcgcaggccg cccagcggca acgcatcgag gcagaagcac 8041 gccccggtga atcgtggcaa gcggccgctg atcgaatccg caaagaatcc cggcaaccgc 8101 cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg cgacgagcaa ccagattttt 8161 tcgttccgat gctctatgac gtgggcaccc gcgatagtcg cagcatcatg gacgtggccg 8221 ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt gatccgctac gagcttccag 8281 acgggcacgt agaggtttcc gcagggccgg ccggcatggc gagtgtgtgg gattacgacc 8341 tggtactgat ggcggtttcc catctaaccg aatccatgaa ccgataccgg gaagggaagg 8401 gagacaagcc cggccgcgtg ttccgtccac acgttgcgga cgtactcaag ttctgccggc 8461 gagccgatgg cggaaagcag aaagacgacc tggtagaaac ctgcattcgg ttaaacacca 8521 cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg ccgcctggtg acggtatccg 8581 agggtgaagc cttgattagc cgctacaaga tcgtaaagag cgaaaccggg cggccggagt 8641 acatcgagat cgagctagct gattggatgt accgcgagat cacagaaggc aagaacccgg 8701 acgtgctgac ggttcacccc gattactttt tgatcgatcc cggcatcggc cgttttctct 8761 accgcctggc acgccgcgcc gcaggcaagg cagaagccag atggttgttc aagacgatct 8821 acgaacgcag tggcagcgcc ggagagttca agaagttctg tttcaccgtg cgcaagctga 8881 tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga ggcggggcag gctggcccga 8941 tcctagtcat gcgctaccgc aacctgatcg agggcgaagc atccgccggt tcctaatgta 9001 cggagcagat gctagggcaa attgccctag caggggaaaa aggtcgaaaa ggtctctttc 9061 ctgtggatag cacgtacatt gggaacccaa agccgtacat tgggaaccgg aacccgtaca 9121 ttgggaaccc aaagccgtac attgggaacc ggtcacacat gtaagtgact gatataaaag 9181 agaaaaaagg cgatttttcc gcctaaaact ctttaaaact tattaaaact cttaaaaccc 9241 gcctggcctg tgcataactg tctggccagc gcacagccga agagctgcaa aaagcgccta 9301 cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg tcggcctatc gcggccgctg 9361 gccgctcaaa aatggctggc ctacggccag gcaatctacc agggcgcgga caagccgcgc 9421 cgtcgccact cgaccgccgg cgcccacatc aaggcaccgg tgggtatgcc tgacgatgcg 9481 tggagaccga aaccttgcgc tcgttcgcca gccaggacag aaatgcctcg acttcgctgc 9541 tgcccaaggt tgccgggtga cgcacaccgt ggaaacggat gaaggcacga acccagtgga 9601 cataagcctg ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt 9661 ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt 9721 atgactgttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg 9781 ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc 9841 ctaaaacaaa gttaaacatc atgagggaag cggtgatcgc cgaagtatcg actcaactat 9901 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt 9961 acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg 10021 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt 10081 cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg 10141 acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc 10201 gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct 10261 tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct 10321 ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga 10381 actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt 10441 ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg 10501 agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac 10561 aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga 10621 aaggcgagat caccaaggta gtcggcaaat aatgtctaac aattcgttca agccgacgcc 10681 gcttcgcggc gcggcttaac tcaagcgtta gatgcactaa gcacataatt gctcacagcc 10741 aaactatcag gtcaagtctg cttttattat ttttaagcgt gcataataag ccctacacaa 10801 attgggagat atatcatgaa aggctggctt tttcttgtta tcgcaatagt tggcgaagta 10861 atcgcaacat agcttgcttg gtcgttccgc gtgaacgtcg gctcgattgt acctgcgttc 10921 aaatactttg cgatcgtgtt gcgcgcctgc ccggtgcgtc ggctgatctc acggatcgac 10981 tgcttctctc gcaacgccat ccgacggatg atgtttaaaa gtcccatgtg gatcactccg 11041 ttgccccgtc gctcaccgtg ttggggggaa ggtgcacatg gctcagttct caatggaaat 11101 tatctgccta accggctcag ttctgcgtag aaaccaacat gcaagctcca ccgggtgcaa 11161 agcggcagcg gcggcaggat atattcaatt gtaaatggct tcatgtccgg gaaatctaca 11221 tggatcagca atgagtatga tggtcaatat ggagaaaaag aaagagtaat taccaatttt 11281 ttttcaattc aaaaatgtag atgtccgcag cgttattata aaatgaaagt acattttgat 11341 aaaacgacaa attacgatcc gtcgtattta taggcgaaag caataaacaa attattctaa 11401 ttcggaaatc tttatttcga cgtgtctaca ttcacgtcca aatgggggct tagatgagaa 11461 acttcacgat cggc //