LOCUS       p7U-35S-RNAi           11637 bp    DNA     circular SYN 13-OCT-2009
DEFINITION  Ligation of inverted 1.pD1-35s-RNAi* into p7U*
ACCESSION   p7U-35S-RNAi
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 11637)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by SciEd Central, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  p7U*
               Fragment ends: SfiI
               Fragment size: 9939
            Insert molecule:  1.pD1-35s-RNAi*
               Fragment ends: SfiI
               Fragment size: 1698
FEATURES             Location/Qualifiers
     misc_feature    10..1517
                     /gene="Ubi-int'"
                     /product="Promotor"
                     /SECDrawAs="Region"
     misc_feature    997..1516
                     /gene="Intron"
                     /product="Intron"
                     /SECDrawAs="Region"
     CDS             1531..2082
                     /gene="bar"
                     /product="Basta-Resistenz"
                     /SECDrawAs="Gene"
     misc_feature    2105..2305
                     /gene="T35S"
                     /product="Terminator"
                     /SECDrawAs="Region"
     misc_feature    2364..2876
                     /gene="Pro-35S"
                     /product="Promotor"
                     /SECDrawAs="Region"
     misc_feature    complement (2890..2938)
                     /gene="T35s"
                     /SECDrawAs="Region"
     CDS             2958..3507
                     /gene="(null)"
                     /SECDrawAs="Gene"
     CDS             complement (2958..3507)
                     /gene="Gus"
                     /SECDrawAs="Gene"
     misc_feature    3528..3576
                     /gene="T35S"
                     /SECDrawAs="Region"
     misc_feature    complement (3589..4010)
                     /gene="Pro-35S"
                     /product="Promotor-Region"
                     /SECDrawAs="Region"
     misc_feature    4112..4135
                     /gene="RB"
                     /product="Right Border"
                     /SECDrawAs="Region"
     misc_feature    4670..5367
                     /gene="ColE1"
                     /product="ColE1 ORI"
                     /SECDrawAs="Region"
     misc_feature    5916..9621
                     /gene="pVS1 ORI"
                     /product="pVS1 ORI"
                     /SECDrawAs="Region"
     CDS             9805..10815
                     /gene="Sm/Sp"
                     /product="Strep/Spec resistence"
                     /SECDrawAs="Gene"
     misc_feature    11336..11359
                     /gene="LB"
                     /product="Left Border"
                     /SECDrawAs="Region"
ORIGIN      
        1 tctagtagtc tgcagtgcag cgtgacccgg tcgtgcccct ctctagagat aatgagcatt
       61 gcatgtctaa gttataaaaa attaccacat attttttttg tcacacttgt ttgaagtgca
      121 gtttatctat ctttatacat atatttaaac tttactctac gaataatata atctatagta
      181 ctacaataat atcagtgttt tagagaatca tataaatgaa cagttagaca tggtctaaag
      241 gacaattgag tattttgaca acaggactct acagttttat ctttttagtg tgcatgtgtt
      301 ctcctttttt tttgcaaata gcttcaccta tataatactt catccatttt attagtacat
      361 ccatttaggg tttagggtta atggttttta tagactaatt tttttagtac atctatttta
      421 ttctatttta gcctctaaat taagaaaact aaaactctat tttagttttt ttatttaata
      481 atttagatat aaaatagaat aaaataaagt gactaaaaat taaacaaata ccctttaaga
      541 aattaaaaaa actaaggaaa catttttctt gtttcgagta gataatgcca gcctgttaaa
      601 cgccgtcgat cgacgagtct aacggacacc aaccagcgaa ccagcagcgt cgcgtcgggc
      661 caagcgaagc agacggcacg gcatctctgt cgctgcctct ggacccctct cgagagttcc
      721 gctccaccgt tggacttgct ccgctgtcgg catccagaaa ttgcgtggcg gagcggcaga
      781 cgtgagccgg cacggcaggc ggcctcctcc tcctctcacg gcaccggcag ctacggggga
      841 ttcctttccc accgctcctt cgctttccct tcctcgcccg ccgtaataaa tagacacccc
      901 ctccacaccc tctttcccca acctcgtgtt gttcggagcg cacacacaca caaccagatc
      961 tcccccaaat ccacccgtcg gcacctccgc ttcaaggtac gccgctcgtc ctcccccccc
     1021 ccccctctct accttctcta gatcggcgtt ccggtccatg gttagggccc ggtagttcta
     1081 cttctgttca tgtttgtgtt agatccgtgt ttgtgttaga tccgtgctgc tagcgttcgt
     1141 acacggatgc gacctgtacg tcagacacgt tctgattgct aacttgccag tgtttctctt
     1201 tggggaatcc tgggatggct ctagccgttc cgcagacggg atcgatctag gataggtata
     1261 catgttgatg tgggttttac tgatgcatat acatgatggc atatgcagca tctattcata
     1321 tgctctaacc ttgagtacct atctattata ataaacaagt atgttttata attattttga
     1381 tcttgatata cttggatgat ggcatatgca gcagctatat gtggattttt ttagccctgc
     1441 cttcatacgc tatttatttg cttggtactg tttcttttgt cgatgctcac cctgttgttt
     1501 ggtgttactt ctgcaggtcg agtggccacc atgggcccag aacgacgccc ggccgacatc
     1561 cgccgtgcca ccgaggcgga catgccggcg gtctgcacca tcgtcaacca ctacatcgag
     1621 acaagcacgg tcaacttccg taccgagccg caggaaccgc aggagtggac ggacgacctc
     1681 gtccgtctgc gggagcgcta tccctggctc gtcgccgagg tggacggcga ggtcgccggc
     1741 atcgcctacg cgggcccctg gaaggcacgc aacgcctacg actggacggc cgagtcgacc
     1801 gtgtacgtct ccccccgcca ccagcggacg ggactgggct ccacgctcta cacccacctg
     1861 ctgaagtccc tggaggcaca gggcttcaag agcgtggtcg ctgtcatcgg gctgcccaac
     1921 gacccgagcg tgcgcatgca cgaggcgctc ggatatgccc cccgcggcat gctgcgggcg
     1981 gccggcttca agcacgggaa ctggcatgac gtgggtttct ggcagctgga cttcagcctg
     2041 ccggtaccgc cccgtccggt cctgcccgtc accgagatct gagatcacgc gttctagtcc
     2101 gcaaaaatca ccagtctctc tctacaaatc tatctctctc tatttttctc cagaataatg
     2161 tgtgagtagt tcccagataa gggaattagg gttcttatag ggtttcgctc atgtgttgag
     2221 catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca ataaaatttc
     2281 taattcctaa aaccaaaatc cagtgacctg caggcatgca agctgatcca ctagaggcca
     2341 tgggggccaa tttaaatggt accaatagag gacctaacag aactcgccgt aaagactggc
     2401 gaacagttca tacagagtct cttacgactc aatgacaaga agaaaatctt cgtcaacatg
     2461 gtggagcacg acacacttgt ctactccaaa aatatcaaag atacagtctc agaagaccaa
     2521 agggcaattg agacttttca acaaagggta atatccggaa acctcctcgg attccattgc
     2581 ccagctatct gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc
     2641 catcattgcg ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa
     2701 gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca
     2761 aagcaagtgg attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat
     2821 ccttcgcaag acccttcctc tatataagga agttcatttc atttggagag aacacggtcg
     2881 actgagctcg ttttaggaat tagagatttt attgagagca gtattttaca aatacaaacg
     2941 cgtctgtaca taagcttcac ccaggtgttc ggcgtggtgt agagcattac gctgcgatgg
     3001 attccggcat agttaaagaa atcatggaag taagactgct ttttcttgcc gttttcgtcg
     3061 gtaatcacca ttcccggcgg gatagtctgc cagttcagtt cgttgttcac acaaacggtg
     3121 atacgtacac ttttcccggc aataacatac ggcgtgacat cggcttcaaa tggcgtatag
     3181 ccgccctgat gctccatcac ttcctgatta ttgacccaca ctttgccgta atgagtgacc
     3241 gcatcgaaac gcagcacgat acgctggcct gcccaacctt tcggtataaa gacttcgcgc
     3301 tgataccaga cgttgcccgc ataattacga atatctgcat cggcgaactg atcgttaaaa
     3361 ctgcctggca cagcaattgc ccggctttct tgtaacgcgc tttcccacca acgctgatca
     3421 attccacagt tttcgcgatc cagactgaat gcccacaggc cgtcgagttt tttgatttca
     3481 cgggttgggg tttctacagg acgtaacctt aagacccggg aggatccttt gtatttgtat
     3541 ttatcagcac acaataaata cacattttcc taaaacggcg ccaactagtg tcctctccaa
     3601 atgaaatgaa cttccttata tagaggaagg gtcttgcgaa ggatagtggg attgtgcgtc
     3661 atcccttacg tcagtggaga tatcacatca atccacttgc tttgaagacg tggttggaac
     3721 gtcttctttt tccacgatgt tcctcgtggg tgggggtcca tctttgggac cactgtcggt
     3781 agaggcatct tgaacgatag cctttccttt atcgcaatga tggcatttgt agaagccatc
     3841 ttccttttct actgtccttt cgatgaagtg acagatagct gggcaatgga atccgaggag
     3901 gtttcccgat attacccttt gttgaaaagt ctcaatagcc ctctggtctt ctgagactgt
     3961 atctttgata ttcttggagt agacgagagt gtcgtgctcc accatgttga tctagtgcgg
     4021 ccgcattaat taaggcccta agggccagat cttgggcccg gtacccgatc agattgtcgt
     4081 ttcccgcctt cggtttaaac tatcagtgtt tgacaggata tattggcggg taaacctaag
     4141 agaaaagagc gtttattaga ataatcggat atttaaaagg gcgtgaaaag gtttatccgt
     4201 tcgtccattt gtatgtgcat gccaaccaca gggttcccct cgggagtgct tggcattccg
     4261 tgcgataatg acttctgttc aaccacccaa acgtcggaaa gcctgacgac ggagcagcat
     4321 tccaaaaaga tcccttggct cgtctgggtc ggctagaagg tcgagtgggc tgctgtggct
     4381 tgatccctca acgcggtcgc ggacgtagcg cagcgccgaa aaatcctcga tcgcaaatcc
     4441 gacgctgtcg aaaagcgtga tctgcttgtc gctctttcgg ccgacgtcct ggccagtcat
     4501 cacgcgccaa agttccgtca caggatgatc tggcgcgagt tgctggatct cgccttcaat
     4561 ccgggtctgt ggcgggaact ccacgaaaat atccgaacgc agcaagatat cgcggtgcat
     4621 ctcggtcttg cctgggcagt cgccgccgac gccgttgatg tggacgccga aaaggatcta
     4681 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     4741 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     4801 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     4861 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     4921 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     4981 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     5041 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     5101 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     5161 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     5221 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     5281 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     5341 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     5401 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     5461 taaccgatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     5521 agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat
     5581 ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca
     5641 tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac
     5701 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     5761 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     5821 aacgcgcgag gcaggggtac gtcgaggtcg atccaacccc tccgctgcta tagtgcagtc
     5881 ggcttctgac gttcagtgca gccgtcttct gaaaacgaca tgtcgcacaa gtcctaagtt
     5941 acgcgacagg ctgccgccct gcccttttcc tggcgttttc ttgtcgcgtg ttttagtcgc
     6001 ataaagtaga atacttgcga ctagaaccgg agacattacg ccatgaacaa gagcgccgcc
     6061 gctggcctgc tgggctatgc ccgcgtcagc accgacgacc aggacttgac caaccaacgg
     6121 gccgaactgc acgcggccgg ctgcaccaag ctgttttccg agaagatcac cggcaccagg
     6181 cgcgaccgcc cggagctggc caggatgctt gaccacctac gccctggcga cgttgtgaca
     6241 gtgaccaggc tagaccgcct ggcccgcagc acccgcgacc tactggacat tgccgagcgc
     6301 atccaggagg ccggcgcggg cctgcgtagc ctggcagagc cgtgggccga caccaccacg
     6361 ccggccggcc gcatggtgtt gaccgtgttc gccggcattg ccgagttcga gcgttcccta
     6421 atcatcgacc gcacccggag cgggcgcgag gccgccaagg cgcgaggcgt gaagtttggc
     6481 ccccgcccta ccctcacccc ggcacagatc gcgcacgccc gcgagctgat cgaccaggaa
     6541 ggccgcaccg tgaaagaggc ggctgcactg cttggcgtgc atcgctcgac cctgtaccgc
     6601 gcacttgagc gcagcgagga agtgacgccc accgaggcca ggcggcgcgg tgccttccgt
     6661 gaggacgcat tgaccgaggc cgacgccctg gcggccgccg agaatgaacg ccaagaggaa
     6721 caagcatgaa accgcaccag gacggccagg acgaaccgtt tttcattacc gaagagatcg
     6781 aggcggagat gatcgcggcc gggtacgtgt tcgagccgcc cgcgcacgtc tcaaccgtgc
     6841 ggctgcatga aatcctggcc ggtttgtctg atgccaagct cgcggcctgg ccggcgagct
     6901 tggccgctga agaaaccgag cgccgccgtc taaaaaggtg atgtgtattt gagtaaaaca
     6961 gcttgcgtca tgcggtcgct gcgtatatga tgcgatgagt aaataaacaa atacgcaagg
     7021 ggaacgcatg aaggttatcg ctgtacttaa ccagaaaggc gggtcaggca agacgaccat
     7081 cgcaacccat ctagcccgcg ccctgcaact cgccggggcc gatgttctgt tagtcgattc
     7141 cgatccccag ggcagtgccc gcgattgggc ggccgtgcgg gaagatcaac cgctaaccgt
     7201 tgtcggcatc gaccgcccga cgattgaccg cgacgtgaag gccatcggcc ggcgcgactt
     7261 cgtagtgatc gacggagcgc cccaggcggc ggacttggct gtgtccgcga tcaaggcagc
     7321 cgacttcgtg ctgattccgg tgcagccaag cccttacgac atatgggcca ccgccgacct
     7381 ggtggagctg gttaagcagc gcattgaggt cacggatgga aggctacaag cggcctttgt
     7441 cgtgtcgcgg gcgatcaaag gcacgcgcat cggcggtgag gttgccgagg cgctggccgg
     7501 gtacgagctg cccattcttg agtcccgtat cacgcagcgc gtgagctacc caggcactgc
     7561 cgccgccggc acaaccgttc ttgaatcaga acccgagggc gacgctgccc gcgaggtcca
     7621 ggcgctggcc gctgaaatta aatcaaaact catttgagtt aatgaggtaa agagaaaatg
     7681 agcaaaagca caaacacgct aagtgccggc cgtccgagcg cacgcagcag caaggctgca
     7741 acgttggcca gcctggcaga cacgccagcc atgaagcggg tcaactttca gttgccggcg
     7801 gaggatcaca ccaagctgaa gatgtacgcg gtacgccaag gcaagaccat taccgagctg
     7861 ctatctgaat acatcgcgca gctaccagag taaatgagca aatgaataaa tgagtagatg
     7921 aattttagcg gctaaaggag gcggcatgga aaatcaagaa caaccaggca ccgacgccgt
     7981 ggaatgcccc atgtgtggag gaacgggcgg ttggccaggc gtaagcggct gggttgtctg
     8041 ccggccctgc aatggcactg gaacccccaa gcccgaggaa tcggcgtgag cggtcgcaaa
     8101 ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg acctggtgga gaagttgaag
     8161 gcggcgcagg ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg tgaatcgtgg
     8221 caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc cggtgcgccg
     8281 tcgattagga agccgcccaa gggcgacgag caaccagatt ttttcgttcc gatgctctat
     8341 gacgtgggca cccgcgatag tcgcagcatc atggacgtgg ccgttttccg tctgtcgaag
     8401 cgtgaccgac gagctggcga ggtgatccgc tacgagcttc cagacgggca cgtagaggtt
     8461 tccgcagggc cggccggcat ggcgagtgtg tgggattacg acctggtact gatggcggtt
     8521 tcccatctaa ccgaatccat gaaccgatac cgggaaggga agggagacaa gcccggccgc
     8581 gtgttccgtc cacacgttgc ggacgtactc aagttctgcc ggcgagccga tggcggaaag
     8641 cagaaagacg acctggtaga aacctgcatt cggttaaaca ccacgcacgt tgccatgcag
     8701 cgtacgaaga aggccaagaa cggccgcctg gtgacggtat ccgagggtga agccttgatt
     8761 agccgctaca agatcgtaaa gagcgaaacc gggcggccgg agtacatcga gatcgagcta
     8821 gctgattgga tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct gacggttcac
     8881 cccgattact ttttgatcga tcccggcatc ggccgttttc tctaccgcct ggcacgccgc
     8941 gccgcaggca aggcagaagc cagatggttg ttcaagacga tctacgaacg cagtggcagc
     9001 gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc aaatgacctg
     9061 ccggagtacg atttgaagga ggaggcgggg caggctggcc cgatcctagt catgcgctac
     9121 cgcaacctga tcgagggcga agcatccgcc ggttcctaat gtacggagca gatgctaggg
     9181 caaattgccc tagcagggga aaaaggtcga aaaggtctct ttcctgtgga tagcacgtac
     9241 attgggaacc caaagccgta cattgggaac cggaacccgt acattgggaa cccaaagccg
     9301 tacattggga accggtcaca catgtaagtg actgatataa aagagaaaaa aggcgatttt
     9361 tccgcctaaa actctttaaa acttattaaa actcttaaaa cccgcctggc ctgtgcataa
     9421 ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc ctacccttcg gtcgctgcgc
     9481 tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg ctggccgctc aaaaatggct
     9541 ggcctacggc caggcaatct accagggcgc ggacaagccg cgccgtcgcc actcgaccgc
     9601 cggcgcccac atcaaggcac cggtgggtat gcctgacgat gcgtggagac cgaaaccttg
     9661 cgctcgttcg ccagccagga cagaaatgcc tcgacttcgc tgctgcccaa ggttgccggg
     9721 tgacgcacac cgtggaaacg gatgaaggca cgaacccagt ggacataagc ctgttcggtt
     9781 cgtaagctgt aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa
     9841 cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttggg
     9901 gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg
     9961 atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac
    10021 atcatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc
    10081 atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat
    10141 ggcggcctga agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat
    10201 gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag
    10261 agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg
    10321 cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca
    10381 ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga
    10441 gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa
    10501 caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg
    10561 gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc
    10621 ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag
    10681 tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg
    10741 gcctcgcgcg cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag
    10801 gtagtcggca aataatgtct aacaattcgt tcaagccgac gccgcttcgc ggcgcggctt
    10861 aactcaagcg ttagatgcac taagcacata attgctcaca gccaaactat caggtcaagt
    10921 ctgcttttat tatttttaag cgtgcataat aagccctaca caaattggga gatatatcat
    10981 gaaaggctgg ctttttcttg ttatcgcaat agttggcgaa gtaatcgcaa catagcttgc
    11041 ttggtcgttc cgcgtgaacg tcggctcgat tgtacctgcg ttcaaatact ttgcgatcgt
    11101 gttgcgcgcc tgcccggtgc gtcggctgat ctcacggatc gactgcttct ctcgcaacgc
    11161 catccgacgg atgatgttta aaagtcccat gtggatcact ccgttgcccc gtcgctcacc
    11221 gtgttggggg gaaggtgcac atggctcagt tctcaatgga aattatctgc ctaaccggct
    11281 cagttctgcg tagaaaccaa catgcaagct ccaccgggtg caaagcggca gcggcggcag
    11341 gatatattca attgtaaatg gcttcatgtc cgggaaatct acatggatca gcaatgagta
    11401 tgatggtcaa tatggagaaa aagaaagagt aattaccaat tttttttcaa ttcaaaaatg
    11461 tagatgtccg cagcgttatt ataaaatgaa agtacatttt gataaaacga caaattacga
    11521 tccgtcgtat ttataggcga aagcaataaa caaattattc taattcggaa atctttattt
    11581 cgacgtgtct acattcacgt ccaaatgggg gcttagatga gaaacttcac gatcggc
//
