LOCUS       p7U10-RNAi             11610 bp    DNA     circular SYN 09-OCT-2009
DEFINITION  Ligation of inverted pD1-35s-RNAi* into p7U10*
ACCESSION   p7U10-RNAi
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 11610)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by SciEd Central, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  p7U10*
               Fragment ends: SfiI
               Fragment size: 9912
            Insert molecule:  pD1-35s-RNAi*
               Fragment ends: SfiI
               Fragment size: 1698
FEATURES             Location/Qualifiers
     misc_feature    7..1484
                     /gene="UBQ10"
                     /product="Promotor UbiQ10 A.thaliana"
                     /SECDrawAs="Region"
     misc_feature    1179..1482
                     /gene="íntron"
                     /SECDrawAs="Region"
     CDS             1504..2055
                     /gene="bar"
                     /product="Basta-Resistenz"
                     /SECDrawAs="Gene"
     misc_feature    2078..2278
                     /gene="T35S"
                     /product="Terminator"
                     /SECDrawAs="Region"
     misc_feature    2337..2849
                     /gene="Pro-35S"
                     /product="Promotor"
                     /SECDrawAs="Region"
     misc_feature    complement (2863..2911)
                     /gene="T35s"
                     /SECDrawAs="Region"
     CDS             2931..3480
                     /gene="(null)"
                     /product="gus"
                     /SECDrawAs="Gene"
     CDS             complement (2931..3480)
                     /gene="Gus"
                     /SECDrawAs="Gene"
     misc_feature    3501..3549
                     /gene="T35S"
                     /SECDrawAs="Region"
     misc_feature    complement (3562..3983)
                     /gene="Pro-35S"
                     /product="Promotor-Region"
                     /SECDrawAs="Region"
     misc_feature    4085..4108
                     /gene="RB"
                     /product="Right Border"
                     /SECDrawAs="Region"
     misc_feature    4643..5340
                     /gene="ColE1"
                     /product="ColE1 ORI"
                     /SECDrawAs="Region"
     misc_feature    5889..9594
                     /gene="pVS1 ORI"
                     /product="pVS1 ORI"
                     /SECDrawAs="Region"
     CDS             9778..10788
                     /gene="Sm/Sp"
                     /product="Strep/Spec resistence"
                     /SECDrawAs="Gene"
     misc_feature    11309..11332
                     /gene="LB"
                     /product="Left Border"
                     /SECDrawAs="Region"
ORIGIN      
        1 tctagtgctt tccttacatt ctgagcctct ttccttctaa tccactcatc tgcatcttct
       61 tgtgtcctta ctaatacctc attggttcca aattccctcc ctttaagcac cagctcgttt
      121 ctgttcttcc acagcctccc aagtatccaa gggactaaag cctccacatt cttcagatca
      181 ggatattctt gtttaagatg ttgaactcta tggaggtttg tatgaactga tgatctagga
      241 ccggataagt tcccttcttc atagcgaact tattcaaaga atgttttgtg tatcattctt
      301 gttacattgt tattaatgaa aaaatattat tggtcattgg actgaacacg agtgttaaat
      361 atggaccagg ccccaaataa gatccattga tatatgaatt aaataacaag aataaatcga
      421 gtcaccaaac cacttgcctt ttttaacgag acttgttcac caacttgata caaaagtcat
      481 tatcctatgc aaatcaataa tcatacaaaa atatccaata acactaaaaa attaaaagaa
      541 atggataatt tcacaatatg ttatacgata aagaagttac ttttccaaga aattcactga
      601 ttttataagc ccacttgcat tagataaatg gcaaaaaaaa acaaaaagga aaagaaataa
      661 agcacgaaga attctagaaa atacgaaata cgcttcaatg cagtgggacc cacggttcaa
      721 ttattgccaa ttttcagctc caccgtatat ttaaaaaata aaacgataat gctaaaaaaa
      781 tataaatcgt aacgatcgtt aaatctcaac ggctggatct tatgacgacc gttagaaatt
      841 gtggttgtcg acgagtcagt aataaacggc gtcaaagtgg ttgcagccgg cacacacgag
      901 tcgtgtttat caactcaaag cacaaatact tttcctcaac ctaaaaataa ggcaattagc
      961 caaaaacaac tttgcgtgta aacaacgctc aatacacgtg tcattttatt attagctatt
     1021 gcttcaccgc cttagctttc tcgtgaccta gtcgtcctcg tcttttcttc ttcttcttct
     1081 ataaaacaat acccaaagag ctcttcttct tcacaattca gatttcaatt tctcaaaatc
     1141 ttaaaaactt tctctcaatt ctctctaccg tgatcaaggt aaatttctgt gttccttatt
     1201 ctctcaaaat cttcgatttt gttttcgttc gatcccaatt tcgtatatgt tctttggttt
     1261 agattctgtt aatcttagat cgaagacgat tttctgggtt tgatcgttag atatcatctt
     1321 aattctcgat tagggtttca tagatatcat ccgatttgtt caaataattt gagttttgtc
     1381 gaataattac tcttcgattt gtgatttcta tctagatctg gtgttagttt ctagtttgtg
     1441 cgatcgaatt tgtcgattaa tctgagtttt tctgattaac agatactagc tcgagtggcc
     1501 accatgggcc cagaacgacg cccggccgac atccgccgtg ccaccgaggc ggacatgccg
     1561 gcggtctgca ccatcgtcaa ccactacatc gagacaagca cggtcaactt ccgtaccgag
     1621 ccgcaggaac cgcaggagtg gacggacgac ctcgtccgtc tgcgggagcg ctatccctgg
     1681 ctcgtcgccg aggtggacgg cgaggtcgcc ggcatcgcct acgcgggccc ctggaaggca
     1741 cgcaacgcct acgactggac ggccgagtcc accgtgtacg tctccccccg ccaccagcgg
     1801 acgggactgg gctccacgct ctacacccac ctgctgaagt ccctggaggc acagggcttc
     1861 aagagcgtgg tcgctgtcat cgggctgccc aacgacccga gcgtgcgcat gcacgaggcg
     1921 ctcggatatg ccccccgcgg catgctgcgg gcggccggct tcaagcacgg gaactggcat
     1981 gacgtgggtt tctggcagct ggacttcagc ctgccggtac cgccccgtcc ggtcctgccc
     2041 gtcaccgaga tctgagatca cgcgttctag tccgcaaaaa tcaccagtct ctctctacaa
     2101 atctatctct ctctattttt ctccagaata atgtgtgagt agttcccaga taagggaatt
     2161 agggttctta tagggtttcg ctcatgtgtt gagcatataa gaaaccctta gtatgtattt
     2221 gtatttgtaa aatacttcta tcaataaaat ttctaattcc taaaaccaaa atccagtgac
     2281 ctgcaggcat gcaagctgat ccactagagg ccatgggggc caatttaaat ggtaccaata
     2341 gaggacctaa cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga
     2401 ctcaatgaca agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc
     2461 aaaaatatca aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg
     2521 gtaatatccg gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag
     2581 atagtggaaa aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc
     2641 gttgaagatg cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc
     2701 gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc
     2761 actgacgtaa gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa
     2821 ggaagttcat ttcatttgga gagaacacgg tcgactgagc tcgttttagg aattagagat
     2881 tttattgaga gcagtatttt acaaatacaa acgcgtctgt acataagctt cacccaggtg
     2941 ttcggcgtgg tgtagagcat tacgctgcga tggattccgg catagttaaa gaaatcatgg
     3001 aagtaagact gctttttctt gccgttttcg tcggtaatca ccattcccgg cgggatagtc
     3061 tgccagttca gttcgttgtt cacacaaacg gtgatacgta cacttttccc ggcaataaca
     3121 tacggcgtga catcggcttc aaatggcgta tagccgccct gatgctccat cacttcctga
     3181 ttattgaccc acactttgcc gtaatgagtg accgcatcga aacgcagcac gatacgctgg
     3241 cctgcccaac ctttcggtat aaagacttcg cgctgatacc agacgttgcc cgcataatta
     3301 cgaatatctg catcggcgaa ctgatcgtta aaactgcctg gcacagcaat tgcccggctt
     3361 tcttgtaacg cgctttccca ccaacgctga tcaattccac agttttcgcg atccagactg
     3421 aatgcccaca ggccgtcgag ttttttgatt tcacgggttg gggtttctac aggacgtaac
     3481 cttaagaccc gggaggatcc tttgtatttg tatttatcag cacacaataa atacacattt
     3541 tcctaaaacg gcgccaacta gtgtcctctc caaatgaaat gaacttcctt atatagagga
     3601 agggtcttgc gaaggatagt gggattgtgc gtcatccctt acgtcagtgg agatatcaca
     3661 tcaatccact tgctttgaag acgtggttgg aacgtcttct ttttccacga tgttcctcgt
     3721 gggtgggggt ccatctttgg gaccactgtc ggtagaggca tcttgaacga tagcctttcc
     3781 tttatcgcaa tgatggcatt tgtagaagcc atcttccttt tctactgtcc tttcgatgaa
     3841 gtgacagata gctgggcaat ggaatccgag gaggtttccc gatattaccc tttgttgaaa
     3901 agtctcaata gccctctggt cttctgagac tgtatctttg atattcttgg agtagacgag
     3961 agtgtcgtgc tccaccatgt tgatctagtg cggccgcatt aattaaggcc ctaagggcca
     4021 gatcttgggc ccggtacccg atcagattgt cgtttcccgc cttcggttta aactatcagt
     4081 gtttgacagg atatattggc gggtaaacct aagagaaaag agcgtttatt agaataatcg
     4141 gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg catgccaacc
     4201 acagggttcc cctcgggagt gcttggcatt ccgtgcgata atgacttctg ttcaaccacc
     4261 caaacgtcgg aaagcctgac gacggagcag cattccaaaa agatcccttg gctcgtctgg
     4321 gtcggctaga aggtcgagtg ggctgctgtg gcttgatccc tcaacgcggt cgcggacgta
     4381 gcgcagcgcc gaaaaatcct cgatcgcaaa tccgacgctg tcgaaaagcg tgatctgctt
     4441 gtcgctcttt cggccgacgt cctggccagt catcacgcgc caaagttccg tcacaggatg
     4501 atctggcgcg agttgctgga tctcgccttc aatccgggtc tgtggcggga actccacgaa
     4561 aatatccgaa cgcagcaaga tatcgcggtg catctcggtc ttgcctgggc agtcgccgcc
     4621 gacgccgttg atgtggacgc cgaaaaggat ctaggtgaag atcctttttg ataatctcat
     4681 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     4741 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     4801 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     4861 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     4921 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     4981 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     5041 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     5101 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     5161 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     5221 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     5281 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     5341 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     5401 gttctttcct gcgttatccc ctgattctgt ggataaccga ttaccgcctt tgagtgagct
     5461 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     5521 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg
     5581 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat
     5641 cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
     5701 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     5761 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagggg tacgtcgagg
     5821 tcgatccaac ccctccgctg ctatagtgca gtcggcttct gacgttcagt gcagccgtct
     5881 tctgaaaacg acatgtcgca caagtcctaa gttacgcgac aggctgccgc cctgcccttt
     5941 tcctggcgtt ttcttgtcgc gtgttttagt cgcataaagt agaatacttg cgactagaac
     6001 cggagacatt acgccatgaa caagagcgcc gccgctggcc tgctgggcta tgcccgcgtc
     6061 agcaccgacg accaggactt gaccaaccaa cgggccgaac tgcacgcggc cggctgcacc
     6121 aagctgtttt ccgagaagat caccggcacc aggcgcgacc gcccggagct ggccaggatg
     6181 cttgaccacc tacgccctgg cgacgttgtg acagtgacca ggctagaccg cctggcccgc
     6241 agcacccgcg acctactgga cattgccgag cgcatccagg aggccggcgc gggcctgcgt
     6301 agcctggcag agccgtgggc cgacaccacc acgccggccg gccgcatggt gttgaccgtg
     6361 ttcgccggca ttgccgagtt cgagcgttcc ctaatcatcg accgcacccg gagcgggcgc
     6421 gaggccgcca aggcgcgagg cgtgaagttt ggcccccgcc ctaccctcac cccggcacag
     6481 atcgcgcacg cccgcgagct gatcgaccag gaaggccgca ccgtgaaaga ggcggctgca
     6541 ctgcttggcg tgcatcgctc gaccctgtac cgcgcacttg agcgcagcga ggaagtgacg
     6601 cccaccgagg ccaggcggcg cggtgccttc cgtgaggacg cattgaccga ggccgacgcc
     6661 ctggcggccg ccgagaatga acgccaagag gaacaagcat gaaaccgcac caggacggcc
     6721 aggacgaacc gtttttcatt accgaagaga tcgaggcgga gatgatcgcg gccgggtacg
     6781 tgttcgagcc gcccgcgcac gtctcaaccg tgcggctgca tgaaatcctg gccggtttgt
     6841 ctgatgccaa gctcgcggcc tggccggcga gcttggccgc tgaagaaacc gagcgccgcc
     6901 gtctaaaaag gtgatgtgta tttgagtaaa acagcttgcg tcatgcggtc gctgcgtata
     6961 tgatgcgatg agtaaataaa caaatacgca aggggaacgc atgaaggtta tcgctgtact
     7021 taaccagaaa ggcgggtcag gcaagacgac catcgcaacc catctagccc gcgccctgca
     7081 actcgccggg gccgatgttc tgttagtcga ttccgatccc cagggcagtg cccgcgattg
     7141 ggcggccgtg cgggaagatc aaccgctaac cgttgtcggc atcgaccgcc cgacgattga
     7201 ccgcgacgtg aaggccatcg gccggcgcga cttcgtagtg atcgacggag cgccccaggc
     7261 ggcggacttg gctgtgtccg cgatcaaggc agccgacttc gtgctgattc cggtgcagcc
     7321 aagcccttac gacatatggg ccaccgccga cctggtggag ctggttaagc agcgcattga
     7381 ggtcacggat ggaaggctac aagcggcctt tgtcgtgtcg cgggcgatca aaggcacgcg
     7441 catcggcggt gaggttgccg aggcgctggc cgggtacgag ctgcccattc ttgagtcccg
     7501 tatcacgcag cgcgtgagct acccaggcac tgccgccgcc ggcacaaccg ttcttgaatc
     7561 agaacccgag ggcgacgctg cccgcgaggt ccaggcgctg gccgctgaaa ttaaatcaaa
     7621 actcatttga gttaatgagg taaagagaaa atgagcaaaa gcacaaacac gctaagtgcc
     7681 ggccgtccga gcgcacgcag cagcaaggct gcaacgttgg ccagcctggc agacacgcca
     7741 gccatgaagc gggtcaactt tcagttgccg gcggaggatc acaccaagct gaagatgtac
     7801 gcggtacgcc aaggcaagac cattaccgag ctgctatctg aatacatcgc gcagctacca
     7861 gagtaaatga gcaaatgaat aaatgagtag atgaatttta gcggctaaag gaggcggcat
     7921 ggaaaatcaa gaacaaccag gcaccgacgc cgtggaatgc cccatgtgtg gaggaacggg
     7981 cggttggcca ggcgtaagcg gctgggttgt ctgccggccc tgcaatggca ctggaacccc
     8041 caagcccgag gaatcggcgt gagcggtcgc aaaccatccg gcccggtaca aatcggcgcg
     8101 gcgctgggtg atgacctggt ggagaagttg aaggcggcgc aggccgccca gcggcaacgc
     8161 atcgaggcag aagcacgccc cggtgaatcg tggcaagcgg ccgctgatcg aatccgcaaa
     8221 gaatcccggc aaccgccggc agccggtgcg ccgtcgatta ggaagccgcc caagggcgac
     8281 gagcaaccag attttttcgt tccgatgctc tatgacgtgg gcacccgcga tagtcgcagc
     8341 atcatggacg tggccgtttt ccgtctgtcg aagcgtgacc gacgagctgg cgaggtgatc
     8401 cgctacgagc ttccagacgg gcacgtagag gtttccgcag ggccggccgg catggcgagt
     8461 gtgtgggatt acgacctggt actgatggcg gtttcccatc taaccgaatc catgaaccga
     8521 taccgggaag ggaagggaga caagcccggc cgcgtgttcc gtccacacgt tgcggacgta
     8581 ctcaagttct gccggcgagc cgatggcgga aagcagaaag acgacctggt agaaacctgc
     8641 attcggttaa acaccacgca cgttgccatg cagcgtacga agaaggccaa gaacggccgc
     8701 ctggtgacgg tatccgaggg tgaagccttg attagccgct acaagatcgt aaagagcgaa
     8761 accgggcggc cggagtacat cgagatcgag ctagctgatt ggatgtaccg cgagatcaca
     8821 gaaggcaaga acccggacgt gctgacggtt caccccgatt actttttgat cgatcccggc
     8881 atcggccgtt ttctctaccg cctggcacgc cgcgccgcag gcaaggcaga agccagatgg
     8941 ttgttcaaga cgatctacga acgcagtggc agcgccggag agttcaagaa gttctgtttc
     9001 accgtgcgca agctgatcgg gtcaaatgac ctgccggagt acgatttgaa ggaggaggcg
     9061 gggcaggctg gcccgatcct agtcatgcgc taccgcaacc tgatcgaggg cgaagcatcc
     9121 gccggttcct aatgtacgga gcagatgcta gggcaaattg ccctagcagg ggaaaaaggt
     9181 cgaaaaggtc tctttcctgt ggatagcacg tacattggga acccaaagcc gtacattggg
     9241 aaccggaacc cgtacattgg gaacccaaag ccgtacattg ggaaccggtc acacatgtaa
     9301 gtgactgata taaaagagaa aaaaggcgat ttttccgcct aaaactcttt aaaacttatt
     9361 aaaactctta aaacccgcct ggcctgtgca taactgtctg gccagcgcac agccgaagag
     9421 ctgcaaaaag cgcctaccct tcggtcgctg cgctccctac gccccgccgc ttcgcgtcgg
     9481 cctatcgcgg ccgctggccg ctcaaaaatg gctggcctac ggccaggcaa tctaccaggg
     9541 cgcggacaag ccgcgccgtc gccactcgac cgccggcgcc cacatcaagg caccggtggg
     9601 tatgcctgac gatgcgtgga gaccgaaacc ttgcgctcgt tcgccagcca ggacagaaat
     9661 gcctcgactt cgctgctgcc caaggttgcc gggtgacgca caccgtggaa acggatgaag
     9721 gcacgaaccc agtggacata agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg
     9781 cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg
     9841 gttttcatgg cttgttatga ctgttttttt ggggtacagt ctatgcctcg ggcatccaag
     9901 cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg
     9961 cagcagggca gtcgccctaa aacaaagtta aacatcatga gggaagcggt gatcgccgaa
    10021 gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg
    10081 ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagtgatatt
    10141 gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac
    10201 gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc
    10261 accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa
    10321 tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac
    10381 attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca
    10441 gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa
    10501 accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt
    10561 acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct
    10621 gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctaga
    10681 caggcttatc ttggacaaga agaagatcgc ttggcctcgc gcgcagatca gttggaagaa
    10741 tttgtccact acgtgaaagg cgagatcacc aaggtagtcg gcaaataatg tctaacaatt
    10801 cgttcaagcc gacgccgctt cgcggcgcgg cttaactcaa gcgttagatg cactaagcac
    10861 ataattgctc acagccaaac tatcaggtca agtctgcttt tattattttt aagcgtgcat
    10921 aataagccct acacaaattg ggagatatat catgaaaggc tggctttttc ttgttatcgc
    10981 aatagttggc gaagtaatcg caacatagct tgcttggtcg ttccgcgtga acgtcggctc
    11041 gattgtacct gcgttcaaat actttgcgat cgtgttgcgc gcctgcccgg tgcgtcggct
    11101 gatctcacgg atcgactgct tctctcgcaa cgccatccga cggatgatgt ttaaaagtcc
    11161 catgtggatc actccgttgc cccgtcgctc accgtgttgg ggggaaggtg cacatggctc
    11221 agttctcaat ggaaattatc tgcctaaccg gctcagttct gcgtagaaac caacatgcaa
    11281 gctccaccgg gtgcaaagcg gcagcggcgg caggatatat tcaattgtaa atggcttcat
    11341 gtccgggaaa tctacatgga tcagcaatga gtatgatggt caatatggag aaaaagaaag
    11401 agtaattacc aatttttttt caattcaaaa atgtagatgt ccgcagcgtt attataaaat
    11461 gaaagtacat tttgataaaa cgacaaatta cgatccgtcg tatttatagg cgaaagcaat
    11521 aaacaaatta ttctaattcg gaaatcttta tttcgacgtg tctacattca cgtccaaatg
    11581 ggggcttaga tgagaaactt cacgatcggc
//
