LOCUS       p9-Nap-RNAi            10588 bp    DNA     circular SYN 25-JUN-2010
DEFINITION  Ligation of inverted 2.pD1-Nap-RNAi* into pLH9000*
ACCESSION   p9-Nap-RNAi
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 10588)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
FEATURES             Location/Qualifiers
     misc_feature    7..422
                     /gene="35S"
                     /product="35S-Promotor"
     CDS             439..1242
                     /gene="NptII"
                     /product="Kanamycin-Resistenz"
     misc_feature    1255..1456
                     /gene="T35S"
                     /product="Terminator 35S"
     misc_feature    1517..1880
                     /gene="Nap"
                     /product="Promotor"
     misc_feature    complement (1899..1947)
                     /gene="T35s"
     CDS             1967..2516
                     /gene="(null)"
     CDS             complement (1967..2516)
                     /gene="Gus"
     misc_feature    2537..2585
                     /gene="T35S"
     misc_feature    complement (2599..2962)
                     /gene="Nap"
                     /product="Promotor"
     misc_feature    3063..3086
                     /gene="BR"
                     /product="Border-right"
     misc_feature    3621..4318
                     /gene="ColE1"
                     /product="ColE1 ORI"
     misc_feature    4355..4614
                     /gene="bom"
     misc_feature    4867..8572
                     /gene="pVS1"
                     /product="pVS1 ori"
     CDS             8756..9766
                     /gene="Sm/Sp"
                     /product="Strep/Spec resistence"
     misc_feature    10287..10310
                     /gene="BL"
                     /product="Border-Left"
ORIGIN      
        1 tctagaacat ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct
       61 cagaagacca gagggctatt gagacttttc aacaaagggt aatatcggga aacctcctcg
      121 gattccattg cccagctatc tgtcacttca tcgaaaggac agtagaaaag gaagatggct
      181 tctacaaatg ccatcattgc gataaaggaa aggctatcgt tcaagatgcc tctaccgaca
      241 gtggtcccaa agatggaccc ccacccacga ggaacatcgt ggaaaaagaa gacgttccaa
      301 ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac
      361 aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga
      421 ggacctcgag tggccaccat gggagcttgg attgaacaag atggattgca cgcaggttct
      481 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc
      541 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc
      601 gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc
      661 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg
      721 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag
      781 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc
      841 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt
      901 cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
      961 gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc
     1021 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg
     1081 ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag
     1141 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg
     1201 cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggacc cgcaaaaatc
     1261 accagtctct ctctacaaat ctatctctct ctatttttct ccagaataat gtgtgagtag
     1321 ttcccagata agggaattag ggttcttata gggtttcgct catgtgttga gcatataaga
     1381 aacccttagt atgtatttgt atttgtaaaa tacttctatc aataaaattt ctaattccta
     1441 aaaccaaaat ccagtgacct gcaggcatgc aagctgatcc actagaggcc atgggggcca
     1501 atttaaatgg taccagaatt tatagactct catccccttt taaaccaact tagtaaacgt
     1561 tttttttttt aattttatga agttaagttt ttaccttgtt tttaaaaaga atcgttcata
     1621 agatgccatg ccagaacatt agctacacgt tacacatagc atgcagccgc ggagaattgt
     1681 ttttcttcgc cacttgtcac tcccttcaaa cacctaagag cttctctctc acagcacaca
     1741 catacaatca catgcgtgca tgcattatta cacgtgatcg ccatgcaaat ctcctttata
     1801 gcctataaat taactcatcc gcttcactct ttactcaaac caaaactcat caatacaaac
     1861 aagattaaaa acatacacga actaggtcga ctgagctcgt tttaggaatt agagatttta
     1921 ttgagagcag tattttacaa atacaaacgc gtctgtacat aagcttcacc caggtgttcg
     1981 gcgtggtgta gagcattacg ctgcgatgga ttccggcata gttaaagaaa tcatggaagt
     2041 aagactgctt tttcttgccg ttttcgtcgg taatcaccat tcccggcggg atagtctgcc
     2101 agttcagttc gttgttcaca caaacggtga tacgtacact tttcccggca ataacatacg
     2161 gcgtgacatc ggcttcaaat ggcgtatagc cgccctgatg ctccatcact tcctgattat
     2221 tgacccacac tttgccgtaa tgagtgaccg catcgaaacg cagcacgata cgctggcctg
     2281 cccaaccttt cggtataaag acttcgcgct gataccagac gttgcccgca taattacgaa
     2341 tatctgcatc ggcgaactga tcgttaaaac tgcctggcac agcaattgcc cggctttctt
     2401 gtaacgcgct ttcccaccaa cgctgatcaa ttccacagtt ttcgcgatcc agactgaatg
     2461 cccacaggcc gtcgagtttt ttgatttcac gggttggggt ttctacagga cgtaacctta
     2521 agacccggga ggatcctttg tatttgtatt tatcagcaca caataaatac acattttcct
     2581 aaaacggcgc caactagttc gtgtatgttt ttaatcttgt ttgtattgat gagttttggt
     2641 ttgagtaaag agtgaagcgg atgagttaat ttataggcta taaaggagat ttgcatggcg
     2701 atcacgtgta ataatgcatg cacgcatgtg attgtatgtg tgtgctgtga gagagaagct
     2761 cttaggtgtt tgaagggagt gacaagtggc gaagaaaaac aattctccgc ggctgcatgc
     2821 tatgtgtaac gtgtagctaa tgttctggca tggcatctta tgaacgattc tttttaaaaa
     2881 caaggtaaaa acttaacttc ataaaattaa aaaaaaaaac gtttactaag ttggtttaaa
     2941 aggggatgag agtctataaa ttctagtgcg gccgcattaa ttaaggccct aagggccaga
     3001 tcttgggccc ggtacccgat cagattgtcg tttcccgcct tcggtttaaa ctatcagtgt
     3061 ttgacaggat atattggcgg gtaaacctaa gagaaaagag cgtttattag aataatcgga
     3121 tatttaaaag ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgca tgccaaccac
     3181 agggttcccc tcgggagtgc ttggcattcc gtgcgataat gacttctgtt caaccaccca
     3241 aacgtcggaa agcctgacga cggagcagca ttccaaaaag atcccttggc tcgtctgggt
     3301 cggctagaag gtcgagtggg ctgctgtggc ttgatccctc aacgcggtcg cggacgtagc
     3361 gcagcgccga aaaatcctcg atcgcaaatc cgacgctgtc gaaaagcgtg atctgcttgt
     3421 cgctctttcg gccgacgtcc tggccagtca tcacgcgcca aagttccgtc acaggatgat
     3481 ctggcgcgag ttgctggatc tcgccttcaa tccgggtctg tggcgggaac tccacgaaaa
     3541 tatccgaacg cagcaagata tcgcggtgca tctcggtctt gcctgggcag tcgccgccga
     3601 cgccgttgat gtggacgccg aaaaggatct aggtgaagat cctttttgat aatctcatga
     3661 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     3721 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     3781 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     3841 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     3901 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     3961 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     4021 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     4081 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     4141 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     4201 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     4261 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     4321 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     4381 tctttcctgc gttatcccct gattctgtgg ataaccgatt accgcctttg agtgagctga
     4441 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     4501 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg
     4561 cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg
     4621 ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga
     4681 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     4741 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcaggggta cgtcgaggtc
     4801 gatccaaccc ctccgctgct atagtgcagt cggcttctga cgttcagtgc agccgtcttc
     4861 tgaaaacgac atgtcgcaca agtcctaagt tacgcgacag gctgccgccc tgcccttttc
     4921 ctggcgtttt cttgtcgcgt gttttagtcg cataaagtag aatacttgcg actagaaccg
     4981 gagacattac gccatgaaca agagcgccgc cgctggcctg ctgggctatg cccgcgtcag
     5041 caccgacgac caggacttga ccaaccaacg ggccgaactg cacgcggccg gctgcaccaa
     5101 gctgttttcc gagaagatca ccggcaccag gcgcgaccgc ccggagctgg ccaggatgct
     5161 tgaccaccta cgccctggcg acgttgtgac agtgaccagg ctagaccgcc tggcccgcag
     5221 cacccgcgac ctactggaca ttgccgagcg catccaggag gccggcgcgg gcctgcgtag
     5281 cctggcagag ccgtgggccg acaccaccac gccggccggc cgcatggtgt tgaccgtgtt
     5341 cgccggcatt gccgagttcg agcgttccct aatcatcgac cgcacccgga gcgggcgcga
     5401 ggccgccaag gcgcgaggcg tgaagtttgg cccccgccct accctcaccc cggcacagat
     5461 cgcgcacgcc cgcgagctga tcgaccagga aggccgcacc gtgaaagagg cggctgcact
     5521 gcttggcgtg catcgctcga ccctgtaccg cgcacttgag cgcagcgagg aagtgacgcc
     5581 caccgaggcc aggcggcgcg gtgccttccg tgaggacgca ttgaccgagg ccgacgccct
     5641 ggcggccgcc gagaatgaac gccaagagga acaagcatga aaccgcacca ggacggccag
     5701 gacgaaccgt ttttcattac cgaagagatc gaggcggaga tgatcgcggc cgggtacgtg
     5761 ttcgagccgc ccgcgcacgt ctcaaccgtg cggctgcatg aaatcctggc cggtttgtct
     5821 gatgccaagc tcgcggcctg gccggcgagc ttggccgctg aagaaaccga gcgccgccgt
     5881 ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg
     5941 atgcgatgag taaataaaca aatacgcaag gggaacgcat gaaggttatc gctgtactta
     6001 accagaaagg cgggtcaggc aagacgacca tcgcaaccca tctagcccgc gccctgcaac
     6061 tcgccggggc cgatgttctg ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg
     6121 cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc
     6181 gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg
     6241 cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt gctgattccg gtgcagccaa
     6301 gcccttacga catatgggcc accgccgacc tggtggagct ggttaagcag cgcattgagg
     6361 tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca
     6421 tcggcggtga ggttgccgag gcgctggccg ggtacgagct gcccattctt gagtcccgta
     6481 tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag
     6541 aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac
     6601 tcatttgagt taatgaggta aagagaaaat gagcaaaagc acaaacacgc taagtgccgg
     6661 ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc agcctggcag acacgccagc
     6721 catgaagcgg gtcaactttc agttgccggc ggaggatcac accaagctga agatgtacgc
     6781 ggtacgccaa ggcaagacca ttaccgagct gctatctgaa tacatcgcgc agctaccaga
     6841 gtaaatgagc aaatgaataa atgagtagat gaattttagc ggctaaagga ggcggcatgg
     6901 aaaatcaaga acaaccaggc accgacgccg tggaatgccc catgtgtgga ggaacgggcg
     6961 gttggccagg cgtaagcggc tgggttgtct gccggccctg caatggcact ggaaccccca
     7021 agcccgagga atcggcgtga gcggtcgcaa accatccggc ccggtacaaa tcggcgcggc
     7081 gctgggtgat gacctggtgg agaagttgaa ggcggcgcag gccgcccagc ggcaacgcat
     7141 cgaggcagaa gcacgccccg gtgaatcgtg gcaagcggcc gctgatcgaa tccgcaaaga
     7201 atcccggcaa ccgccggcag ccggtgcgcc gtcgattagg aagccgccca agggcgacga
     7261 gcaaccagat tttttcgttc cgatgctcta tgacgtgggc acccgcgata gtcgcagcat
     7321 catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga cgagctggcg aggtgatccg
     7381 ctacgagctt ccagacgggc acgtagaggt ttccgcaggg ccggccggca tggcgagtgt
     7441 gtgggattac gacctggtac tgatggcggt ttcccatcta accgaatcca tgaaccgata
     7501 ccgggaaggg aagggagaca agcccggccg cgtgttccgt ccacacgttg cggacgtact
     7561 caagttctgc cggcgagccg atggcggaaa gcagaaagac gacctggtag aaacctgcat
     7621 tcggttaaac accacgcacg ttgccatgca gcgtacgaag aaggccaaga acggccgcct
     7681 ggtgacggta tccgagggtg aagccttgat tagccgctac aagatcgtaa agagcgaaac
     7741 cgggcggccg gagtacatcg agatcgagct agctgattgg atgtaccgcg agatcacaga
     7801 aggcaagaac ccggacgtgc tgacggttca ccccgattac tttttgatcg atcccggcat
     7861 cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc aaggcagaag ccagatggtt
     7921 gttcaagacg atctacgaac gcagtggcag cgccggagag ttcaagaagt tctgtttcac
     7981 cgtgcgcaag ctgatcgggt caaatgacct gccggagtac gatttgaagg aggaggcggg
     8041 gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg atcgagggcg aagcatccgc
     8101 cggttcctaa tgtacggagc agatgctagg gcaaattgcc ctagcagggg aaaaaggtcg
     8161 aaaaggtctc tttcctgtgg atagcacgta cattgggaac ccaaagccgt acattgggaa
     8221 ccggaacccg tacattggga acccaaagcc gtacattggg aaccggtcac acatgtaagt
     8281 gactgatata aaagagaaaa aaggcgattt ttccgcctaa aactctttaa aacttattaa
     8341 aactcttaaa acccgcctgg cctgtgcata actgtctggc cagcgcacag ccgaagagct
     8401 gcaaaaagcg cctacccttc ggtcgctgcg ctccctacgc cccgccgctt cgcgtcggcc
     8461 tatcgcggcc gctggccgct caaaaatggc tggcctacgg ccaggcaatc taccagggcg
     8521 cggacaagcc gcgccgtcgc cactcgaccg ccggcgccca catcaaggca ccggtgggta
     8581 tgcctgacga tgcgtggaga ccgaaacctt gcgctcgttc gccagccagg acagaaatgc
     8641 ctcgacttcg ctgctgccca aggttgccgg gtgacgcaca ccgtggaaac ggatgaaggc
     8701 acgaacccag tggacataag cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg
     8761 ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg cagtggcggt
     8821 tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg catccaagca
     8881 gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg atgttacgca
     8941 gcagggcagt cgccctaaaa caaagttaaa catcatgagg gaagcggtga tcgccgaagt
     9001 atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac cgacgttgct
     9061 ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca gtgatattga
     9121 tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt tgatcaacga
     9181 ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg tagaagtcac
     9241 cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg aactgcaatt
     9301 tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca cgatcgacat
     9361 tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg taggtccagc
     9421 ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc taaatgaaac
     9481 cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg tagtgcttac
     9541 gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc
     9601 cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg aagctagaca
     9661 ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt
     9721 tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataatgtc taacaattcg
     9781 ttcaagccga cgccgcttcg cggcgcggct taactcaagc gttagatgca ctaagcacat
     9841 aattgctcac agccaaacta tcaggtcaag tctgctttta ttatttttaa gcgtgcataa
     9901 taagccctac acaaattggg agatatatca tgaaaggctg gctttttctt gttatcgcaa
     9961 tagttggcga agtaatcgca acatagcttg cttggtcgtt ccgcgtgaac gtcggctcga
    10021 ttgtacctgc gttcaaatac tttgcgatcg tgttgcgcgc ctgcccggtg cgtcggctga
    10081 tctcacggat cgactgcttc tctcgcaacg ccatccgacg gatgatgttt aaaagtccca
    10141 tgtggatcac tccgttgccc cgtcgctcac cgtgttgggg ggaaggtgca catggctcag
    10201 ttctcaatgg aaattatctg cctaaccggc tcagttctgc gtagaaacca acatgcaagc
    10261 tccaccgggt gcaaagcggc agcggcggca ggatatattc aattgtaaat ggcttcatgt
    10321 ccgggaaatc tacatggatc agcaatgagt atgatggtca atatggagaa aaagaaagag
    10381 taattaccaa ttttttttca attcaaaaat gtagatgtcc gcagcgttat tataaaatga
    10441 aagtacattt tgataaaacg acaaattacg atccgtcgta tttataggcg aaagcaataa
    10501 acaaattatt ctaattcgga aatctttatt tcgacgtgtc tacattcacg tccaaatggg
    10561 ggcttagatg agaaacttca cgatcggc
//
