LOCUS p9i-UbiH-2xStp-R 10741 bp DNA circular SYN 20-JUN-2013 DEFINITION Ligation of inverted A252pD1-UbiH-2xStp-RNAi* into p9i* ACCESSION p9i-UbiH-2xStp-R KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 10741) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: p9i* Fragment ends: SfiI Fragment size: 9279 Insert molecule: A252pD1-UbiH-2xStp-RNAi* Fragment ends: SfiI Fragment size: 1460 Sequencing Primer: KRB CTGATAGTTTAAACCGAAGGC T35F CGCTCATGTGTTGAGCATATAA Sequencing Primer: SphR GGGTGGTTGAACAGAAGTCAT RNAi3R GTTGGCGCCGTTTTAGGAAAAT T35F CGCTCATGTGTTGAGCATATAA FEATURES Location/Qualifiers misc_feature 7..422 /gene="35S" /product="35S-Promotor" /SECDrawAs="Region" /SECStyleId=1 /SECName="35S" /SECDescr="35S-Promotor" CDS 439..475 /gene="NptII" /product="Kanamycin resistance" /SECDrawAs="Gene" /SECStyleId=1 /SECName="NptII" /SECDescr="Kanamycin resistance" misc_feature 476..664 /gene="int" /product="Intron StLS1" /SECDrawAs="Gene" /SECStyleId=1 /SECName="int" /SECDescr="Intron StLS1" CDS 665..1431 /gene="NptII" /product="Kanamycin-Resistenz" /SECDrawAs="Gene" /SECStyleId=1 /SECName="NptII" /SECDescr="Kanamycin-Resistenz" misc_feature 1444..1645 /gene="T-35S" /product="Terminator 35S" /SECDrawAs="Region" /SECStyleId=1 /SECName="T-35S" /SECDescr="Terminator 35S" misc_feature 1704..2047 /gene="UbiH" /product="Promotor aus Sonnenblume" /SECDrawAs="Region" /SECStyleId=1 misc_feature complement(2062..2114) /gene="T35S" /SECDrawAs="Region" /SECStyleId=1 CDS 2134..2683 /SECDrawAs="Gene" /SECStyleId=1 CDS complement(2134..2683) /gene="Gus" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 2704..2756 /gene="T35S" /SECDrawAs="Region" /SECStyleId=1 misc_feature complement(2771..3114) /gene="UbiH" /product="Promotor aus Sonnenblume" /SECDrawAs="Region" /SECStyleId=1 misc_feature 3216..3239 /gene="BR" /product="Border-right" /SECDrawAs="Region" /SECStyleId=1 /SECName="BR" /SECDescr="Border-right" misc_feature 3774..4471 /gene="ColE1" /product="ColE1 ORI" /SECDrawAs="Region" /SECStyleId=1 /SECName="ColE1" /SECDescr="ColE1 ORI" misc_feature 5020..8725 /gene="pVS1 ORI" /product="pVS1 ORI" /SECDrawAs="Region" /SECStyleId=1 /SECName="pVS1 ORI" /SECDescr="pVS1 ORI" CDS 8909..9919 /gene="Sm/Sp" /product="Strep/Spec resistence" /SECDrawAs="Gene" /SECStyleId=1 /SECName="Sm/Sp" /SECDescr="Strep/Spec resistence" misc_feature 10440..10463 /gene="BL" /product="Border-Left" /SECDrawAs="Region" /SECStyleId=1 /SECName="BL" /SECDescr="Border-Left" ORIGIN 1 tctagaacat ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct 61 cagaagacca gagggctatt gagacttttc aacaaagggt aatatcggga aacctcctcg 121 gattccattg cccagctatc tgtcacttca tcgaaaggac agtagaaaag gaagatggct 181 tctacaaatg ccatcattgc gataaaggaa aggctatcgt tcaagatgcc tctaccgaca 241 gtggtcccaa agatggaccc ccacccacga ggaacatcgt ggaaaaagaa gacgttccaa 301 ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac 361 aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga 421 ggacctcgag tggccaccat gggagcttgg attgaacaag atggattgca cgcaggtaag 481 tttctgcttc tacctttgat atatatataa taattatcat taattagtag taatataata 541 tttcaaatat ttttttcaaa ataaaagaat gtagtatata gcaattgctt ttctgtagtt 601 tataagtgtg tatattttaa tttataactt ttctaatata tgaccaaaat ttgttgatgt 661 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca 721 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt 781 gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg 841 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga 901 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct 961 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg 1021 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg 1081 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc 1141 gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat 1201 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac 1261 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt 1321 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct 1381 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggaccc 1441 gcaaaaatca ccagtctctc tctacaaatc tatctctctc tatttttctc cagaataatg 1501 tgtgagtagt tcccagataa gggaattagg gttcttatag ggtttcgctc atgtgttgag 1561 catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca ataaaatttc 1621 taattcctaa aaccaaaatc cagtgacctg caggcatgca agctgatcca ctagaggcca 1681 tgggggccaa tttaaatggt accgtcgaca aaacttaaga aagtcaccaa aaagaaagtc 1741 aaagacggca acggcaaccc agaagctacg ttctctttct agattctatg ccctacctac 1801 cgcgtggtgc acaacaactt gacccccatc attgactctc caccgtacac gtgtcacgat 1861 accatttgtt ggtctcctac cacttacgta acattaatat tccatccgtg accgcctctt 1921 cttcatcatc ctataaattc accttcttat ctcttccgct ttcattcgtt catcatcatc 1981 acaattaaca acaatccaaa gcaatcttct ctcatattct tatctcttcc tgtttcgctt 2041 cgtcttgggt cgactgagct cgttttagga attagagatt ttatttattg agagcagtat 2101 tttacaaata caaacgcgtc tgtacataag cttcacccag gtgttcggcg tggtgtagag 2161 cattacgctg cgatggattc cggcatagtt aaagaaatca tggaagtaag actgcttttt 2221 cttgccgttt tcgtcggtaa tcaccattcc cggcgggata gtctgccagt tcagttcgtt 2281 gttcacacaa acggtgatac gtacactttt cccggcaata acatacggcg tgacatcggc 2341 ttcaaatggc gtatagccgc cctgatgctc catcacttcc tgattattga cccacacttt 2401 gccgtaatga gtgaccgcat cgaaacgcag cacgatacgc tggcctgccc aacctttcgg 2461 tataaagact tcgcgctgat accagacgtt gcccgcataa ttacgaatat ctgcatcggc 2521 gaactgatcg ttaaaactgc ctggcacagc aattgcccgg ctttcttgta acgcgctttc 2581 ccaccaacgc tgatcaattc cacagttttc gcgatccaga ctgaatgccc acaggccgtc 2641 gagttttttg atttcacggg ttggggtttc tacaggacgt aaccttaaga cccgggagga 2701 tcctttgtat ttgtatttat cagcacacaa taaataaata cacattttcc taaaacggcg 2761 ccaactagtc caagacgaag cgaaacagga agagataaga atatgagaga agattgcttt 2821 ggattgttgt taattgtgat gatgatgaac gaatgaaagc ggaagagata agaaggtgaa 2881 tttataggat gatgaagaag aggcggtcac ggatggaata ttaatgttac gtaagtggta 2941 ggagaccaac aaatggtatc gtgacacgtg tacggtggag agtcaatgat gggggtcaag 3001 ttgttgtgca ccacgcggta ggtagggcat agaatctaga aagagaacgt agcttctggg 3061 ttgccgttgc cgtctttgac tttctttttg gtgactttct taagttttgt cgactctagt 3121 gcggccgcat taattaaggc cctaagggcc agatcttggg cccggtaccc gatcagattg 3181 tcgtttcccg ccttcggttt aaactatcag tgtttgacag gatatattgg cgggtaaacc 3241 taagagaaaa gagcgtttat tagaataatc ggatatttaa aagggcgtga aaaggtttat 3301 ccgttcgtcc atttgtatgt gcatgccaac cacagggttc ccctcgggag tgcttggcat 3361 tccgtgcgat aatgacttct gttcaaccac ccaaacgtcg gaaagcctga cgacggagca 3421 gcattccaaa aagatccctt ggctcgtctg ggtcggctag aaggtcgagt gggctgctgt 3481 ggcttgatcc ctcaacgcgg tcgcggacgt agcgcagcgc cgaaaaatcc tcgatcgcaa 3541 atccgacgct gtcgaaaagc gtgatctgct tgtcgctctt tcggccgacg tcctggccag 3601 tcatcacgcg ccaaagttcc gtcacaggat gatctggcgc gagttgctgg atctcgcctt 3661 caatccgggt ctgtggcggg aactccacga aaatatccga acgcagcaag atatcgcggt 3721 gcatctcggt cttgcctggg cagtcgccgc cgacgccgtt gatgtggacg ccgaaaagga 3781 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt 3841 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc 3901 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc 3961 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac 4021 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac 4081 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt 4141 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct 4201 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat 4261 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt 4321 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg 4381 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt 4441 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt 4501 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg 4561 tggataaccg attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga 4621 gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac 4681 gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc 4741 cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc 4801 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct 4861 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca 4921 ccgaaacgcg cgaggcaggg gtacgtcgag gtcgatccaa cccctccgct gctatagtgc 4981 agtcggcttc tgacgttcag tgcagccgtc ttctgaaaac gacatgtcgc acaagtccta 5041 agttacgcga caggctgccg ccctgccctt ttcctggcgt tttcttgtcg cgtgttttag 5101 tcgcataaag tagaatactt gcgactagaa ccggagacat tacgccatga acaagagcgc 5161 cgccgctggc ctgctgggct atgcccgcgt cagcaccgac gaccaggact tgaccaacca 5221 acgggccgaa ctgcacgcgg ccggctgcac caagctgttt tccgagaaga tcaccggcac 5281 caggcgcgac cgcccggagc tggccaggat gcttgaccac ctacgccctg gcgacgttgt 5341 gacagtgacc aggctagacc gcctggcccg cagcacccgc gacctactgg acattgccga 5401 gcgcatccag gaggccggcg cgggcctgcg tagcctggca gagccgtggg ccgacaccac 5461 cacgccggcc ggccgcatgg tgttgaccgt gttcgccggc attgccgagt tcgagcgttc 5521 cctaatcatc gaccgcaccc ggagcgggcg cgaggccgcc aaggcgcgag gcgtgaagtt 5581 tggcccccgc cctaccctca ccccggcaca gatcgcgcac gcccgcgagc tgatcgacca 5641 ggaaggccgc accgtgaaag aggcggctgc actgcttggc gtgcatcgct cgaccctgta 5701 ccgcgcactt gagcgcagcg aggaagtgac gcccaccgag gccaggcggc gcggtgcctt 5761 ccgtgaggac gcattgaccg aggccgacgc cctggcggcc gccgagaatg aacgccaaga 5821 ggaacaagca tgaaaccgca ccaggacggc caggacgaac cgtttttcat taccgaagag 5881 atcgaggcgg agatgatcgc ggccgggtac gtgttcgagc cgcccgcgca cgtctcaacc 5941 gtgcggctgc atgaaatcct ggccggtttg tctgatgcca agctcgcggc ctggccggcg 6001 agcttggccg ctgaagaaac cgagcgccgc cgtctaaaaa ggtgatgtgt atttgagtaa 6061 aacagcttgc gtcatgcggt cgctgcgtat atgatgcgat gagtaaataa acaaatacgc 6121 aaggggaacg catgaaggtt atcgctgtac ttaaccagaa aggcgggtca ggcaagacga 6181 ccatcgcaac ccatctagcc cgcgccctgc aactcgccgg ggccgatgtt ctgttagtcg 6241 attccgatcc ccagggcagt gcccgcgatt gggcggccgt gcgggaagat caaccgctaa 6301 ccgttgtcgg catcgaccgc ccgacgattg accgcgacgt gaaggccatc ggccggcgcg 6361 acttcgtagt gatcgacgga gcgccccagg cggcggactt ggctgtgtcc gcgatcaagg 6421 cagccgactt cgtgctgatt ccggtgcagc caagccctta cgacatatgg gccaccgccg 6481 acctggtgga gctggttaag cagcgcattg aggtcacgga tggaaggcta caagcggcct 6541 ttgtcgtgtc gcgggcgatc aaaggcacgc gcatcggcgg tgaggttgcc gaggcgctgg 6601 ccgggtacga gctgcccatt cttgagtccc gtatcacgca gcgcgtgagc tacccaggca 6661 ctgccgccgc cggcacaacc gttcttgaat cagaacccga gggcgacgct gcccgcgagg 6721 tccaggcgct ggccgctgaa attaaatcaa aactcatttg agttaatgag gtaaagagaa 6781 aatgagcaaa agcacaaaca cgctaagtgc cggccgtccg agcgcacgca gcagcaaggc 6841 tgcaacgttg gccagcctgg cagacacgcc agccatgaag cgggtcaact ttcagttgcc 6901 ggcggaggat cacaccaagc tgaagatgta cgcggtacgc caaggcaaga ccattaccga 6961 gctgctatct gaatacatcg cgcagctacc agagtaaatg agcaaatgaa taaatgagta 7021 gatgaatttt agcggctaaa ggaggcggca tggaaaatca agaacaacca ggcaccgacg 7081 ccgtggaatg ccccatgtgt ggaggaacgg gcggttggcc aggcgtaagc ggctgggttg 7141 tctgccggcc ctgcaatggc actggaaccc ccaagcccga ggaatcggcg tgagcggtcg 7201 caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt 7261 gaaggcggcg caggccgccc agcggcaacg catcgaggca gaagcacgcc ccggtgaatc 7321 gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg caaccgccgg cagccggtgc 7381 gccgtcgatt aggaagccgc ccaagggcga cgagcaacca gattttttcg ttccgatgct 7441 ctatgacgtg ggcacccgcg atagtcgcag catcatggac gtggccgttt tccgtctgtc 7501 gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag cttccagacg ggcacgtaga 7561 ggtttccgca gggccggccg gcatggcgag tgtgtgggat tacgacctgg tactgatggc 7621 ggtttcccat ctaaccgaat ccatgaaccg ataccgggaa gggaagggag acaagcccgg 7681 ccgcgtgttc cgtccacacg ttgcggacgt actcaagttc tgccggcgag ccgatggcgg 7741 aaagcagaaa gacgacctgg tagaaacctg cattcggtta aacaccacgc acgttgccat 7801 gcagcgtacg aagaaggcca agaacggccg cctggtgacg gtatccgagg gtgaagcctt 7861 gattagccgc tacaagatcg taaagagcga aaccgggcgg ccggagtaca tcgagatcga 7921 gctagctgat tggatgtacc gcgagatcac agaaggcaag aacccggacg tgctgacggt 7981 tcaccccgat tactttttga tcgatcccgg catcggccgt tttctctacc gcctggcacg 8041 ccgcgccgca ggcaaggcag aagccagatg gttgttcaag acgatctacg aacgcagtgg 8101 cagcgccgga gagttcaaga agttctgttt caccgtgcgc aagctgatcg ggtcaaatga 8161 cctgccggag tacgatttga aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg 8221 ctaccgcaac ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg agcagatgct 8281 agggcaaatt gccctagcag gggaaaaagg tcgaaaaggt ctctttcctg tggatagcac 8341 gtacattggg aacccaaagc cgtacattgg gaaccggaac ccgtacattg ggaacccaaa 8401 gccgtacatt gggaaccggt cacacatgta agtgactgat ataaaagaga aaaaaggcga 8461 tttttccgcc taaaactctt taaaacttat taaaactctt aaaacccgcc tggcctgtgc 8521 ataactgtct ggccagcgca cagccgaaga gctgcaaaaa gcgcctaccc ttcggtcgct 8581 gcgctcccta cgccccgccg cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat 8641 ggctggccta cggccaggca atctaccagg gcgcggacaa gccgcgccgt cgccactcga 8701 ccgccggcgc ccacatcaag gcaccggtgg gtatgcctga cgatgcgtgg agaccgaaac 8761 cttgcgctcg ttcgccagcc aggacagaaa tgcctcgact tcgctgctgc ccaaggttgc 8821 cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc cagtggacat aagcctgttc 8881 ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac 8941 cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt 9001 tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg 9061 tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt 9121 aaacatcatg agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg 9181 cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt 9241 ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct 9301 tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg 9361 agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc 9421 gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct 9481 tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc 9541 aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc 9601 tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga 9661 ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt 9721 aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc 9781 ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg 9841 cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac 9901 caaggtagtc ggcaaataat gtctaacaat tcgttcaagc cgacgccgct tcgcggcgcg 9961 gcttaactca agcgttagat gcactaagca cataattgct cacagccaaa ctatcaggtc 10021 aagtctgctt ttattatttt taagcgtgca taataagccc tacacaaatt gggagatata 10081 tcatgaaagg ctggcttttt cttgttatcg caatagttgg cgaagtaatc gcaacatagc 10141 ttgcttggtc gttccgcgtg aacgtcggct cgattgtacc tgcgttcaaa tactttgcga 10201 tcgtgttgcg cgcctgcccg gtgcgtcggc tgatctcacg gatcgactgc ttctctcgca 10261 acgccatccg acggatgatg tttaaaagtc ccatgtggat cactccgttg ccccgtcgct 10321 caccgtgttg gggggaaggt gcacatggct cagttctcaa tggaaattat ctgcctaacc 10381 ggctcagttc tgcgtagaaa ccaacatgca agctccaccg ggtgcaaagc ggcagcggcg 10441 gcaggatata ttcaattgta aatggcttca tgtccgggaa atctacatgg atcagcaatg 10501 agtatgatgg tcaatatgga gaaaaagaaa gagtaattac caattttttt tcaattcaaa 10561 aatgtagatg tccgcagcgt tattataaaa tgaaagtaca ttttgataaa acgacaaatt 10621 acgatccgtc gtatttatag gcgaaagcaa taaacaaatt attctaattc ggaaatcttt 10681 atttcgacgt gtctacattc acgtccaaat gggggcttag atgagaaact tcacgatcgg 10741 c //