LOCUS pD1-35s-Ubi10-RN 4372 bp DNA circular SYN 20-JUN-2013 DEFINITION Ligation of 204pUbiAtmu-ocs* into pD1-35s-RNAi* ACCESSION pD1-35s-Ubi10-RN KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 4372) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: pD1-35s-RNAi* Fragment ends: SpeI and NotI Fragment size: 2889 Insert molecule: 204pUbiAtmu-ocs* Fragment ends: NotI and SpeI Fragment size: 1483 FEATURES Location/Qualifiers misc_feature 71..1546 /gene="Pro-Ubq10At" /SECDrawAs="Region" /SECStyleId=1 CDS 1175..1238 /gene="Ubi cds 5`" /product="Ubi cds 5`" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 1241..1544 /gene="int" /product="intron Ubq10At" /SECDrawAs="Region" /SECStyleId=1 misc_feature complement(1560..1608) /gene="T35S" /SECDrawAs="Region" /SECStyleId=1 CDS 1629..2178 /gene="Gus" /SECDrawAs="Gene" /SECStyleId=1 CDS complement(1629..2178) /SECDrawAs="Gene" /SECStyleId=1 misc_feature 2198..2246 /gene="T35s" /SECDrawAs="Region" /SECStyleId=1 misc_feature complement(2260..2772) /gene="Pro-35S" /product="Promotor" /SECDrawAs="Region" /SECStyleId=1 CDS 2855..3717 /gene="Amp" /product="Amp-DCS1" /SECDrawAs="Gene" /SECStyleId=1 misc_feature join(3718..4372,1..10) /gene="ColE1" /product="ColE1" /SECDrawAs="Region" /SECStyleId=1 ORIGIN 1 cctatggaaa aacgccagca agcggccttt atacagatca ggcccttagg gccttaatta 61 atgcggccgc tttccttaca ttctgagcct ctttccttct aatccactca tctgcatctt 121 cttgtgtcct tactaatacc tcattggttc caaattccct ccctttaagc accagctcgt 181 ttctgttctt ccacagcctc ccaagtatcc aagggactaa agcctccaca ttcttcagat 241 caggatattc ttgtttaaga tgttgaactc tatggaggtt tgtatgaact gatgatctag 301 gaccggataa gttcccttct tcatagcgaa cttattcaaa gaatgttttg tgtatcattc 361 ttgttacatt gttattaatg aaaaaatatt attggtcatt ggactgaaca cgagtgttaa 421 atatggacca ggccccaaat aagatccatt gatatatgaa ttaaataaca agaataaatc 481 gagtcaccaa accacttgcc ttttttaacg agacttgttc accaacttga tacaaaagtc 541 attatcctat gcaaatcaat aatcatacaa aaatatccaa taacactaaa aaattaaaag 601 aaatggataa tttcacaata tgttatacga taaagaagtt acttttccaa gaaattcact 661 gattttataa gcccacttgc attagataaa tggcaaaaaa aaacaaaaag gaaaagaaat 721 aaagcacgaa gaattctaga aaatacgaaa tacgcttcaa tgcagtggga cccacggttc 781 aattattgcc aattttcagc tccaccgtat atttaaaaaa taaaacgata atgctaaaaa 841 aatataaatc gtaacgatcg ttaaatctca acggctggat cttatgacga ccgttagaaa 901 ttgtggttgt cgacgagtca gtaataaacg gcgtcaaagt ggttgcagcc ggcacacacg 961 agtcgtgttt atcaactcaa agcacaaata cttttcctca acctaaaaat aaggcaatta 1021 gccaaaaaca actttgcgtg taaacaacgc tcaatacacg tgtcatttta ttattagcta 1081 ttgcttcacc gccttagctt tctcgtgacc tagtcgtcct cgtcttttct tcttcttctt 1141 ctataaaaca atacccaaag agctcttctt cttcacaatt cagatttcaa tttctcaaaa 1201 tcttaaaaac tttctctcaa ttctctctac cgtgatcaag gtaaatttct gtgttcctta 1261 ttctctcaaa atcttcgatt ttgttttcgt tcgatcccaa tttcgtatat gttctttggt 1321 ttagattctg ttaatcttag atcgaagacg attttctggg tttgatcgtt agatatcatc 1381 ttaattctcg attagggttt catagatatc atccgatttg ttcaaataat ttgagttttg 1441 tcgaataatt actcttcgat ttgtgatttc tatctagatc tggtgttagt ttctagtttg 1501 tgcgatcgaa tttgtcgatt aatctgagtt tttctgatta acaggtacta gttggcgccg 1561 ttttaggaaa atgtgtattt attgtgtgct gataaataca aatacaaagg atcctcccgg 1621 gtcttaaggt tacgtcctgt agaaacccca acccgtgaaa tcaaaaaact cgacggcctg 1681 tgggcattca gtctggatcg cgaaaactgt ggaattgatc agcgttggtg ggaaagcgcg 1741 ttacaagaaa gccgggcaat tgctgtgcca ggcagtttta acgatcagtt cgccgatgca 1801 gatattcgta attatgcggg caacgtctgg tatcagcgcg aagtctttat accgaaaggt 1861 tgggcaggcc agcgtatcgt gctgcgtttc gatgcggtca ctcattacgg caaagtgtgg 1921 gtcaataatc aggaagtgat ggagcatcag ggcggctata cgccatttga agccgatgtc 1981 acgccgtatg ttattgccgg gaaaagtgta cgtatcaccg tttgtgtgaa caacgaactg 2041 aactggcaga ctatcccgcc gggaatggtg attaccgacg aaaacggcaa gaaaaagcag 2101 tcttacttcc atgatttctt taactatgcc ggaatccatc gcagcgtaat gctctacacc 2161 acgccgaaca cctgggtgaa gcttatgtac agacgcgttt gtatttgtaa aatactgctc 2221 tcaataaaat ctctaattcc taaaacgagc tcagtcgacc gtgttctctc caaatgaaat 2281 gaacttcctt atatagagga agggtcttgc gaaggatagt gggattgtgc gtcatccctt 2341 acgtcagtgg agatatcaca tcaatccact tgctttgaag acgtggttgg aacgtcttct 2401 ttttccacga tgctcctcgt gggtgggggt ccatctttgg gaccactgtc ggcagaggca 2461 tcttcaacga tggcctttcc tttatcgcaa tgatggcatt tgtaggagcc accttccttt 2521 tccactatct tcacaataaa gtgacagata gctgggcaat ggaatccgag gaggtttccg 2581 gatattaccc tttgttgaaa agtctcaatt gccctttggt cttctgagac tgtatctttg 2641 atatttttgg agtagacaag tgtgtcgtgc tccaccatgt tgacgaagat tttcttcttg 2701 tcattgagtc gtaagagact ctgtatgaac tgttcgccag tctttacggc gagttctgtt 2761 aggtcctcta ttggtaccat ttaaattggc ccccaaggcc tgatcttgag agctattcta 2821 gtcactcata taatgtctgc ataggaggtc agccatgagt attcaacatt tccgtgtcgc 2881 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt 2941 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct 3001 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac 3061 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact 3121 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa 3181 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga 3241 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt 3301 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga 3361 agccatacca aacgacgagc gtgacaccac gatgccagca gcaatggcaa caacgttgcg 3421 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat 3481 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat 3541 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc 3601 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga 3661 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtgatctca 3721 tggccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga 3781 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa 3841 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga 3901 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt 3961 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt 4021 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat 4081 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct 4141 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca 4201 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag 4261 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc 4321 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg ag //