LOCUS       pD1-Ubi-Act-RNAi        5260 bp    DNA     circular SYN 20-JUN-2013
DEFINITION  Ligation of inverted 153pD1-Act-RNAi* into 46pD1-Ubi-L-RNAi*
ACCESSION   pD1-Ubi-Act-RNAi
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 5260)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  46pD1-Ubi-L-RNAi*
               Fragment ends: KpnI and SalI
               Fragment size: 3848
            Insert molecule:  153pD1-Act-RNAi*
               Fragment ends: KpnI and SalI
               Fragment size: 1404
            GELR
FEATURES             Location/Qualifiers
     misc_feature    71..1546
                     /gene="UbiZm"
                     /product="Promotor Ubi Zea mays"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    1025..1544
                     /gene="int"
                     /product="Intron UbiZm"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    complement(1559..1607)
                     /gene="T35S"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             1628..2177
                     /gene="Gus"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             complement(1628..2177)
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    2197..2245
                     /gene="T35s"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    complement(2263..3660)
                     /gene="pAct"
                     /product="Actin Promotor"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             3743..4605
                     /gene="Amp"
                     /product="Amp-DCS1"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    join(4606..5260,1..10)
                     /gene="ColE1"
                     /product="ColE1"
                     /SECDrawAs="Region"
                     /SECStyleId=1
ORIGIN      
        1 cctatggaaa aacgccagca agcggccttt atacagatca ggcccttagg gccttaatta
       61 atgcggccgc ctagagataa tgagcattgc atgtctaagt tataaaaaat taccacatat
      121 tttttttgtc acacttgttt gaagtgcagt ttatctatct ttatacatat atttaaactt
      181 tactctacga ataatataat ctatagtact acaataatat cagtgtttta gagaatcata
      241 taaatgaaca gttagacatg gtctaaagga caattgagta ttttgacaac aggactctac
      301 agttttatct ttttagtgtg catgtgttct cctttttttt tgcaaatagc ttcacctata
      361 taatacttca tccattttat tagtacatcc atttagggtt tagggttaat ggtttttata
      421 gactaatttt tttagtacat ctattttatt ctattttagc ctctaaatta agaaaactaa
      481 aactctattt tagttttttt atttaataat ttagatataa aatagaataa aataaagtga
      541 ctaaaaatta aacaaatacc ctttaagaaa ttaaaaaaac taaggaaaca tttttcttgt
      601 ttcgagtaga taatgccagc ctgttaaacg ccgtcgatcg acgagtctaa cggacaccaa
      661 ccagcgaacc agcagcgtcg cgtcgggcca agcgaagcag acggcacggc atctctgtcg
      721 ctgcctctgg acccctctcg agagttccgc tccaccgttg gacttgctcc gctgtcggca
      781 tccagaaatt gcgtggcgga gcggcagacg tgagccggca cggcaggcgg cctcctcctc
      841 ctctcacggc accggcagct acgggggatt cctttcccac cgctccttcg ctttcccttc
      901 ctcgcccgcc gtaataaata gacaccccct ccacaccctc tttccccaac ctcgtgttgt
      961 tcggagcgca cacacacaca accagatctc ccccaaatcc acccgtcggc acctccgctt
     1021 caaggtacgc cgctcgtcct cccccccccc ccctctctac cttctctaga tcggcgttcc
     1081 ggtccatggt tagggcccgg tagttctact tctgttcatg tttgtgttag atccgtgttt
     1141 gtgttagatc cgtgctgcta gcgttcgtac acggatgcga cctgtacgtc agacacgttc
     1201 tgattgctaa cttgccagtg tttctctttg gggaatcctg ggatggctct agccgttccg
     1261 cagacgggat cgatctagga taggtataca tgttgatgtg ggttttactg atgcatatac
     1321 atgatggcat atgcagcatc tattcatatg ctctaacctt gagtacctat ctattataat
     1381 aaacaagtat gttttataat tattttgatc ttgatatact tggatgatgg catatgcagc
     1441 agctatatgt ggattttttt agccctgcct tcatacgcta tttatttgct tggtactgtt
     1501 tcttttgtcg atgctcaccc tgttgtttgg tgttacttct gcaggactag ttggcgccgt
     1561 tttaggaaaa tgtgtattta ttgtgtgctg ataaatacaa atacaaagga tcctcccggg
     1621 tcttaaggtt acgtcctgta gaaaccccaa cccgtgaaat caaaaaactc gacggcctgt
     1681 gggcattcag tctggatcgc gaaaactgtg gaattgatca gcgttggtgg gaaagcgcgt
     1741 tacaagaaag ccgggcaatt gctgtgccag gcagttttaa cgatcagttc gccgatgcag
     1801 atattcgtaa ttatgcgggc aacgtctggt atcagcgcga agtctttata ccgaaaggtt
     1861 gggcaggcca gcgtatcgtg ctgcgtttcg atgcggtcac tcattacggc aaagtgtggg
     1921 tcaataatca ggaagtgatg gagcatcagg gcggctatac gccatttgaa gccgatgtca
     1981 cgccgtatgt tattgccggg aaaagtgtac gtatcaccgt ttgtgtgaac aacgaactga
     2041 actggcagac tatcccgccg ggaatggtga ttaccgacga aaacggcaag aaaaagcagt
     2101 cttacttcca tgatttcttt aactatgccg gaatccatcg cagcgtaatg ctctacacca
     2161 cgccgaacac ctgggtgaag cttatgtaca gacgcgtttg tatttgtaaa atactgctct
     2221 caataaaatc tctaattcct aaaacgagct cagtcgacct agtacctaca aaaaagctcc
     2281 gcacgaggct gcatttgtca caaatcatga aaagaaaaac taccgatgaa caatgctgag
     2341 ggattcaaat tctacccaca aaaagaagaa agaaagatct agcacatcta agcctgacga
     2401 agcagcagaa atatataaaa atataaacca tagtgccctt ttcccctctt cctgatcttg
     2461 tttagcatgg cggaaatttt aaacccccca tcatctcccc caacaacggc ggatcgcaga
     2521 tctacatccg agagccccat tccccgcgag atccgggccg gatccacgcc ggcgagagcc
     2581 ccagccgcga gatcccgccc ctcccgcgca ccgatctggg cgcgcacgaa gccgcctctc
     2641 gcccacccaa actaccaagg ccaaagatcg agaccgagac ggaaaaaaaa aacggagaaa
     2701 gaaagaggag aggggcgggg tggttaccgg cgcggcggcg gcggaggggg aggggggagg
     2761 agctcgtcgt ccggcagcga ggggggagga ggtggaggtg gtggtggtgg tggtggtagg
     2821 gttgggggga tgggaggaga ggggggggta tgtatatagt ggcgatgggg ggcgtttctt
     2881 tggaagcgga gggagggccg gcctcgtcgc tggctcgcga tcctcctcgc gtttccggcc
     2941 cccacgaccc ggacccacct gctgtttttt ctttttcttt tttttctttc tttttttttt
     3001 tttggctgcg agacgtgcgg tgcgtgcgga caactcacgg tgatagtggg ggggtgtgga
     3061 gactattgtc cagttggctg gactggggtg ggttgggttg ggttgggttg ggctgggctt
     3121 gctatggatc gtggatagca ctttgggctt taggaacttt aggggttgtt tttgtaaatg
     3181 ttttgagtct aagtttatct tttattttta ctagaaaaaa tacccatgcg ctgcaacggg
     3241 ggaaagctat tttaatctta ttattgttca ttgtgagaat tcgcctgaat atatattttt
     3301 ctcaaaaatt atgtcaaatt agcatatggg tttttttaaa gatatttctt atacaaatcc
     3361 ctctgtattt acaaaagcaa acgaacttaa aacccgactc aaatacagat atgcatttcc
     3421 aaaagcgaat aaacttaaaa accaattcat acaaaaatga cgtatcaaag taccgacaaa
     3481 aacatcctca atttttataa tagtagaaaa gagtaaattt cactttgggc caccttttat
     3541 taccgatatt ttactttata ccacctttta actgatgttt tcacttttga ccaggtaatc
     3601 ttacctttgt tttattttgg actatcccga ctctcttctc aagcatatga atgacctcga
     3661 ggtaccattt aaattggccc ccaaggcctg atcttgagag ctattctagt cactcatata
     3721 atgtctgcat aggaggtcag ccatgagtat tcaacatttc cgtgtcgccc ttattccctt
     3781 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     3841 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     3901 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     3961 gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat
     4021 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     4081 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     4141 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     4201 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     4261 cgacgagcgt gacaccacga tgccagcagc aatggcaaca acgttgcgca aactattaac
     4321 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     4381 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     4441 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     4501 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     4561 acagatcgct gagataggtg cctcactgat taagcattgg tgatctcatg gccaaaatcc
     4621 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     4681 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     4741 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     4801 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     4861 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     4921 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     4981 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     5041 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     5101 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     5161 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     5221 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
//
