LOCUS       A287pD1-Ubi-RNAi        5338 bp    DNA     circular SYN 13-FEB-2013
DEFINITION  Ligation of inverted Ubi-Spe-Eag* into 44pD1-35s-Ubi-RNAi*
ACCESSION   A287pD1-Ubi-RNAi
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 5338)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
FEATURES             Location/Qualifiers
     CDS             73..1548
                     /gene="'Ubi-int'"
                     /product="Promotor"
     misc_feature    1027..1546
                     /gene="Intron"
                     /product="Intron"
     misc_feature    complement(1562..1610)
                     /gene="T35S"
     CDS             1631..2180
                     /gene="Gus"
     CDS             complement(1631..2180)
     misc_feature    2200..2248
                     /gene="T35s"
     CDS             complement(2262..3737)
                     /gene="'Ubi-int'"
                     /product="Promotor"
     misc_feature    complement(2264..2783)
                     /gene="Intron"
                     /product="Intron"
     CDS             3821..4683
                     /gene="Amp"
                     /product="Amp-DCS1"
     misc_feature    join(4684..5338,1..10)
                     /gene="ColE1"
                     /product="ColE1"
ORIGIN      
        1 cctatggaaa aacgccagca agcggccttt atacagatca ggcccttagg gccttaatta
       61 atgcggccgc ctctagagat aatgagcatt gcatgtctaa gttataaaaa attaccacat
      121 attttttttg tcacacttgt ttgaagtgca gtttatctat ctttatacat atatttaaac
      181 tttactctac gaataatata atctatagta ctacaataat atcagtgttt tagagaatca
      241 tataaatgaa cagttagaca tggtctaaag gacaattgag tattttgaca acaggactct
      301 acagttttat ctttttagtg tgcatgtgtt ctcctttttt tttgcaaata gcttcaccta
      361 tataatactt catccatttt attagtacat ccatttaggg tttagggtta atggttttta
      421 tagactaatt tttttagtac atctatttta ttctatttta gcctctaaat taagaaaact
      481 aaaactctat tttagttttt ttatttaata atttagatat aaaatagaat aaaataaagt
      541 gactaaaaat taaacaaata ccctttaaga aattaaaaaa actaaggaaa catttttctt
      601 gtttcgagta gataatgcca gcctgttaaa cgccgtcgat cgacgagtct aacggacacc
      661 aaccagcgaa ccagcagcgt cgcgtcgggc caagcgaagc agacggcacg gcatctctgt
      721 cgctgcctct ggacccctct cgagagttcc gctccaccgt tggacttgct ccgctgtcgg
      781 catccagaaa ttgcgtggcg gagcggcaga cgtgagccgg cacggcaggc ggcctcctcc
      841 tcctctcacg gcaccggcag ctacggggga ttcctttccc accgctcctt cgctttccct
      901 tcctcgcccg ccgtaataaa tagacacccc ctccacaccc tctttcccca acctcgtgtt
      961 gttcggagcg cacacacaca caaccagatc tcccccaaat ccacccgtcg gcacctccgc
     1021 ttcaaggtac gccgctcgtc ctcccccccc ccccctctct accttctcta gatcggcgtt
     1081 ccggtccatg gttagggccc ggtagttcta cttctgttca tgtttgtgtt agatccgtgt
     1141 ttgtgttaga tccgtgctgc tagcgttcgt acacggatgc gacctgtacg tcagacacgt
     1201 tctgattgct aacttgccag tgtttctctt tggggaatcc tgggatggct ctagccgttc
     1261 cgcagacggg atcgatctag gataggtata catgttgatg tgggttttac tgatgcatat
     1321 acatgatggc atatgcagca tctattcata tgctctaacc ttgagtacct atctattata
     1381 ataaacaagt atgttttata attattttga tcttgatata cttggatgat ggcatatgca
     1441 gcagctatat gtggattttt ttagccctgc cttcatacgc tatttatttg cttggtactg
     1501 tttcttttgt cgatgctcac cctgttgttt ggtgttactt ctgcaggtac tagttggcgc
     1561 cgttttagga aaatgtgtat ttattgtgtg ctgataaata caaatacaaa ggatcctccc
     1621 gggtcttaag gttacgtcct gtagaaaccc caacccgtga aatcaaaaaa ctcgacggcc
     1681 tgtgggcatt cagtctggat cgcgaaaact gtggaattga tcagcgttgg tgggaaagcg
     1741 cgttacaaga aagccgggca attgctgtgc caggcagttt taacgatcag ttcgccgatg
     1801 cagatattcg taattatgcg ggcaacgtct ggtatcagcg cgaagtcttt ataccgaaag
     1861 gttgggcagg ccagcgtatc gtgctgcgtt tcgatgcggt cactcattac ggcaaagtgt
     1921 gggtcaataa tcaggaagtg atggagcatc agggcggcta tacgccattt gaagccgatg
     1981 tcacgccgta tgttattgcc gggaaaagtg tacgtatcac cgtttgtgtg aacaacgaac
     2041 tgaactggca gactatcccg ccgggaatgg tgattaccga cgaaaacggc aagaaaaagc
     2101 agtcttactt ccatgatttc tttaactatg ccggaatcca tcgcagcgta atgctctaca
     2161 ccacgccgaa cacctgggtg aagcttatgt acagacgcgt ttgtatttgt aaaatactgc
     2221 tctcaataaa atctctaatt cctaaaacga gctcagtcga cacctgcaga agtaacacca
     2281 aacaacaggg tgagcatcga caaaagaaac agtaccaagc aaataaatag cgtatgaagg
     2341 cagggctaaa aaaatccaca tatagctgct gcatatgcca tcatccaagt atatcaagat
     2401 caaaataatt ataaaacata cttgtttatt ataatagata ggtactcaag gttagagcat
     2461 atgaatagat gctgcatatg ccatcatgta tatgcatcag taaaacccac atcaacatgt
     2521 atacctatcc tagatcgatc ccgtctgcgg aacggctaga gccatcccag gattccccaa
     2581 agagaaacac tggcaagtta gcaatcagaa cgtgtctgac gtacaggtcg catccgtgta
     2641 cgaacgctag cagcacggat ctaacacaaa cacggatcta acacaaacat gaacagaagt
     2701 agaactaccg ggccctaacc atggaccgga acgccgatct agagaaggta gagagggggg
     2761 gggggggagg acgagcggcg taccttgaag cggaggtgcc gacgggtgga tttgggggag
     2821 atctggttgt gtgtgtgtgc gctccgaaca acacgaggtt ggggaaagag ggtgtggagg
     2881 gggtgtctat ttattacggc gggcgaggaa gggaaagcga aggagcggtg ggaaaggaat
     2941 cccccgtagc tgccggtgcc gtgagaggag gaggaggccg cctgccgtgc cggctcacgt
     3001 ctgccgctcc gccacgcaat ttctggatgc cgacagcgga gcaagtccaa cggtggagcg
     3061 gaactctcga gaggggtcca gaggcagcga cagagatgcc gtgccgtctg cttcgcttgg
     3121 cccgacgcga cgctgctggt tcgctggttg gtgtccgtta gactcgtcga tcgacggcgt
     3181 ttaacaggct ggcattatct actcgaaaca agaaaaatgt ttccttagtt tttttaattt
     3241 cttaaagggt atttgtttaa tttttagtca ctttatttta ttctatttta tatctaaatt
     3301 attaaataaa aaaactaaaa tagagtttta gttttcttaa tttagaggct aaaatagaat
     3361 aaaatagatg tactaaaaaa attagtctat aaaaaccatt aaccctaaac cctaaatgga
     3421 tgtactaata aaatggatga agtattatat aggtgaagct atttgcaaaa aaaaaggaga
     3481 acacatgcac actaaaaaga taaaactgta gagtcctgtt gtcaaaatac tcaattgtcc
     3541 tttagaccat gtctaactgt tcatttatat gattctctaa aacactgata ttattgtagt
     3601 actatagatt atattattcg tagagtaaag tttaaatata tgtataaaga tagataaact
     3661 gcacttcaaa caagtgtgac aaaaaaaata tgtggtaatt ttttataact tagacatgca
     3721 atgctcatta tctctagagg taccatttaa attggccccc aaggcctgat cttgagagct
     3781 attctagtca ctcatataat gtctgcatag gaggtcagcc atgagtattc aacatttccg
     3841 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     3901 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     3961 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     4021 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga
     4081 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     4141 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     4201 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     4261 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     4321 gaatgaagcc ataccaaacg acgagcgtga caccacgatg ccagcagcaa tggcaacaac
     4381 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     4441 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     4501 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     4561 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     4621 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggtg
     4681 atctcatggc caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     4741 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     4801 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     4861 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     4921 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     4981 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     5041 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     5101 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     5161 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     5221 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     5281 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggag
//
