LOCUS       pLN35-Gus-RNAi          4761 bp    DNA     circular SYN 13-JUL-2010
DEFINITION  Ligation of GusAS into pLN35-GusS-SG
ACCESSION   pLN35-Gus-RNAi
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 4761)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by SciEd Central, Scientific & Educational Software
FEATURES             Location/Qualifiers
     misc_feature    46..79
                     /gene="loxP"
                     /product="LoxP511"
     misc_feature    95..513
                     /gene="P35s"
                     /product="Promotor-Region"
     CDS             521..1070
                     /gene="GUS-sense"
                     /product="GUS-sense"
     misc_feature    1072..2073
                     /gene="Spacer-GUS"
                     /product="Spacer Gus"
     CDS             complement (2121..2672)
                     /gene="GUS-AS"
                     /product="GUS-Antisense"
     misc_feature    2686..2939
                     /gene="Tnos"
                     /product="NOS-T"
     misc_feature    2946..2979
                     /gene="LoxP"
                     /product="LoxP511"
     misc_feature    complement (3029..3695)
                     /gene="ColE1"
                     /product="ColE1"
     CDS             complement (3745..4605)
                     /gene="Amp"
                     /product="Amp"
ORIGIN      
        1 catttccccg aaaagtgcca ctagaggccc ttagggcctt aattaataac ttcgtataat
       61 gtatactata cgaagttatt gcggccgcac tagatcaaca tggtggagca cgacactctc
      121 gtctactcca agaatatcaa agatacagtc tcagaagacc agagggctat tgagactttt
      181 caacaaaggg taatatcggg aaacctcctc ggattccatt gcccagctat ctgtcacttc
      241 atcgaaagga cagtagaaaa ggaagatggc ttctacaaat gccatcattg cgataaagga
      301 aaggctatcg ttcaagaatg cctctaccga cagtggtccc aaagatggac ccccacccac
      361 gaggaacatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg
      421 tgatatctcc actgacgtaa gggatgacgc acaatcccac tatccttcgc aagacccttc
      481 ctctatataa ggaagttcat ttcatttgga gaggacacta gttacgtcct gtagaaaccc
      541 caacccgtga aatcaaaaaa ctcgacggcc tgtgggcatt cagtctggat cgcgaaaact
      601 gtggaattga tcagcgttgg tgggaaagcg cgttacaaga aagccgggca attgctgtgc
      661 caggcagttt taacgatcag ttcgccgatg cagatattcg taattatgcg ggcaacgtct
      721 ggtatcagcg cgaagtcttt ataccgaaag gttgggcagg ccagcgtatc gtgctgcgtt
      781 tcgatgcggt cactcattac ggcaaagtgt gggtcaataa tcaggaagtg atggagcatc
      841 agggcggcta tacgccattt gaagccgatg tcacgccgta tgttattgcc gggaaaagtg
      901 tacgtatcac cgtttgtgtg aacaacgaac tgaactggca gactatcccg ccgggaatgg
      961 tgattaccga cgaaaacggc aagaaaaagc agtcttactt ccatgatttc tttaactatg
     1021 ccggaatcca tcgcagcgta atgctctaca ccacgccgaa cacctgggtg gatccggtca
     1081 gtggcagtga agggcgaaca gttcctgatt aaccacaaac cgttctactt tactggcttt
     1141 ggtcgtcatg aagatgcgga cttgcgtggc aaaggattcg ataacgtgct gatggtgcac
     1201 gaccacgcat taatggactg gattggggcc aactcctacc gtacctcgca ttacccttac
     1261 gctgaagaga tgctcgactg ggcagatgaa catggcatcg tggtgattga tgaaactgct
     1321 gctgtcggct ttaacctctc tttaggcatt ggtttcgaag cgggcaacaa gccgaaagaa
     1381 ctgtacagcg aagaggcagt caacggggaa actcagcaag cgcacttaca ggcgattaaa
     1441 gagctgatag cgcgtgacaa aaaccaccca agcgtggtga tgtggagtat tgccaacgaa
     1501 ccggataccc gtccgcaagg tgcacgggaa tatttcgcgc cactggcgga agcaacgcgt
     1561 aaactcgacc cgacgcgtcc gatcacctgc gtcaatgtaa tgttctgcga cgctcacacc
     1621 gataccatca gcgatctctt tgatgtgctg tgcctgaacc gttattacgg atggtatgtc
     1681 caaagcggcg atttggaaac ggcagagaag gtactggaaa aagaacttct ggcctggcag
     1741 gagaaactgc atcagccgat tatcatcacc gaatacggcg tggatacgtt agccgggctg
     1801 cactcaatgt acaccgacat gtggagtgaa gagtatcagt gtgcatggct ggatatgtat
     1861 caccgcgtct ttgatcgcgt cagcgccgtc gtcggtgaac aggtatggaa tttcgccgat
     1921 tttgcgacct cgcaaggcat attgcgcgtt ggcggtaaca agaaagggat cttcactcgc
     1981 gaccgcaaac cgaagtcggc ggcttttctg ctgcaaaaac gctggactgg catgaacttc
     2041 ggtgaaaaac cgcagcaggg aggcaaacaa tgaatcaaca actctcctgg cgcaccatcg
     2101 tcggctacag cctcgggaat tccacccagg tgttcggcgt ggtgtagagc attacgctgc
     2161 gatggattcc ggcatagtta aagaaatcat ggaagtaaga ctgctttttc ttgccgtttt
     2221 cgtcggtaat caccattccc ggcgggatag tctgccagtt cagttcgttg ttcacacaaa
     2281 cggtgatacg tacacttttc ccggcaataa catacggcgt gacatcggct tcaaatggcg
     2341 tatagccgcc ctgatgctcc atcacttcct gattattgac ccacactttg ccgtaatgag
     2401 tgaccgcatc gaaacgcagc acgatacgct ggcctgccca acctttcggt ataaagactt
     2461 cgcgctgata ccagacgttg cccgcataat tacgaatatc tgcatcggcg aactgatcgt
     2521 taaaactgcc tggcacagca attgcccggc tttcttgtaa cgcgctttcc caccaacgct
     2581 gatcaattcc acagttttcg cgatccagac tgaatgccca caggccgtcg agttttttga
     2641 tttcacgggt tggggtttct acaggacgta acgagctcag tcgacgatcg ttcaaacatt
     2701 tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa
     2761 tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg
     2821 agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa
     2881 atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcgg
     2941 tacctataac ttcgtataat gtatactata cgaagttatt taaattggcc cccaaggcct
     3001 ctagtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     3061 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     3121 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     3181 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     3241 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     3301 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     3361 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     3421 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     3481 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     3541 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     3601 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     3661 acgaaaactc acgttaaggg attttggcca tgagacaata accctgataa atgcttcaat
     3721 aatattgaaa aaggaagacc atgattacca atgcttaatc agtgaggcac ctatctcagc
     3781 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     3841 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     3901 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     3961 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     4021 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct gctggcatcg tggtgtcacg
     4081 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     4141 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     4201 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     4261 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     4321 atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc
     4381 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     4441 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     4501 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     4561 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatggctg tcagaccaag
     4621 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     4681 tgaagatcct ttttgataat ctcatgggcg gatacatatt tgaatgtatt tagaaaaata
     4741 aacaaatagg ggttccgcgc a
//
