LOCUS       pLN35-SG                3680 bp    DNA     circular SYN 13-JUL-2010
DEFINITION  Ligation of Spacer-Gus into pLN35
ACCESSION   pLN35-SG
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by SciEd Central, Scientific & Educational Software
FEATURES             Location/Qualifiers
     misc_feature    46..79
                     /gene="loxP"
                     /product="LoxP511"
     misc_feature    95..513
                     /gene="P35s"
                     /product="Promotor-Region"
     misc_feature    526..1527
                     /gene="Spacer-GUS"
                     /product="GUS"
     misc_feature    1605..1858
                     /gene="NOS"
                     /product="NOS-T"
     misc_feature    1865..1898
                     /gene="LoxP"
                     /product="LoxP511"
     misc_feature    complement (1948..2614)
                     /gene="ColE1"
                     /product="ColE1"
     CDS             complement (2664..3524)
                     /gene="Amp"
                     /product="Amp"
ORIGIN      
        1 catttccccg aaaagtgcca ctagaggccc ttagggcctt aattaataac ttcgtataat
       61 gtatactata cgaagttatt gcggccgcac tagatcaaca tggtggagca cgacactctc
      121 gtctactcca agaatatcaa agatacagtc tcagaagacc agagggctat tgagactttt
      181 caacaaaggg taatatcggg aaacctcctc ggattccatt gcccagctat ctgtcacttc
      241 atcgaaagga cagtagaaaa ggaagatggc ttctacaaat gccatcattg cgataaagga
      301 aaggctatcg ttcaagaatg cctctaccga cagtggtccc aaagatggac ccccacccac
      361 gaggaacatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg
      421 tgatatctcc actgacgtaa gggatgacgc acaatcccac tatccttcgc aagacccttc
      481 ctctatataa ggaagttcat ttcatttgga gaggacacta gttggatccg gtcagtggca
      541 gtgaagggcg aacagttcct gattaaccac aaaccgttct actttactgg ctttggtcgt
      601 catgaagatg cggacttgcg tggcaaagga ttcgataacg tgctgatggt gcacgaccac
      661 gcattaatgg actggattgg ggccaactcc taccgtacct cgcattaccc ttacgctgaa
      721 gagatgctcg actgggcaga tgaacatggc atcgtggtga ttgatgaaac tgctgctgtc
      781 ggctttaacc tctctttagg cattggtttc gaagcgggca acaagccgaa agaactgtac
      841 agcgaagagg cagtcaacgg ggaaactcag caagcgcact tacaggcgat taaagagctg
      901 atagcgcgtg acaaaaacca cccaagcgtg gtgatgtgga gtattgccaa cgaaccggat
      961 acccgtccgc aaggtgcacg ggaatatttc gcgccactgg cggaagcaac gcgtaaactc
     1021 gacccgacgc gtccgatcac ctgcgtcaat gtaatgttct gcgacgctca caccgatacc
     1081 atcagcgatc tctttgatgt gctgtgcctg aaccgttatt acggatggta tgtccaaagc
     1141 ggcgatttgg aaacggcaga gaaggtactg gaaaaagaac ttctggcctg gcaggagaaa
     1201 ctgcatcagc cgattatcat caccgaatac ggcgtggata cgttagccgg gctgcactca
     1261 atgtacaccg acatgtggag tgaagagtat cagtgtgcat ggctggatat gtatcaccgc
     1321 gtctttgatc gcgtcagcgc cgtcgtcggt gaacaggtat ggaatttcgc cgattttgcg
     1381 acctcgcaag gcatattgcg cgttggcggt aacaagaaag ggatcttcac tcgcgaccgc
     1441 aaaccgaagt cggcggcttt tctgctgcaa aaacgctgga ctggcatgaa cttcggtgaa
     1501 aaaccgcagc agggaggcaa acaatgaatc aacaactctc ctggcgcacc atcgtcggct
     1561 acagcctcgg gaattccaag cttggcgcgc cgagctcagt cgacgatcgt tcaaacattt
     1621 ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt atcatataat
     1681 ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg ttatttatga
     1741 gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata gaaaacaaaa
     1801 tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta ctagatcggt
     1861 acctataact tcgtataatg tatactatac gaagttattt aaattggccc ccaaggcctc
     1921 tagtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     1981 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     2041 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     2101 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     2161 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     2221 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     2281 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     2341 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     2401 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     2461 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     2521 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     2581 cgaaaactca cgttaaggga ttttggccat gagacaataa ccctgataaa tgcttcaata
     2641 atattgaaaa aggaagacca tgattaccaa tgcttaatca gtgaggcacc tatctcagcg
     2701 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     2761 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     2821 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     2881 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     2941 tcgccagtta atagtttgcg caacgttgtt gccattgctg ctggcatcgt ggtgtcacgc
     3001 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     3061 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     3121 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     3181 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     3241 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca
     3301 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     3361 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     3421 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     3481 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatggctgt cagaccaagt
     3541 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     3601 gaagatcctt tttgataatc tcatgggcgg atacatattt gaatgtattt agaaaaataa
     3661 acaaataggg gttccgcgca
//
