LOCUS       pLNU-SG                 4495 bp    DNA     circular SYN 13-JUL-2010
DEFINITION  Ligation of Spacer-Gus into Lox-Ubi-Nos
ACCESSION   pLNU-SG
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 4495)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by SciEd Central, Scientific & Educational Software
FEATURES             Location/Qualifiers
     misc_feature    13..46
                     /gene="loxP"
                     /product="LoxP511"
     misc_feature    62..1570
                     /gene="Ubi-int"
                     /product="Promotor"
     misc_feature    1049..1568
                     /gene="Intron"
                     /product="Intron"
     misc_feature    1581..2353
                     /gene="SG-Gli"
                     /product="GUS"
     misc_feature    2387..2640
                     /gene="NOS"
                     /product="NOS-Terminator"
     misc_feature    2647..2680
                     /gene="LoxP"
                     /product="LoxP511"
     misc_feature    complement (2730..3396)
                     /gene="ColE1"
                     /product="ColE1"
     CDS             complement (3446..4306)
                     /gene="Amp"
                     /product="Amp-DCS1'"
ORIGIN      
        1 gggccttaat taataacttc gtataatgta tactatacga agttattgcg gccgcactag
       61 gctgcagtgc agcgtgaccc ggtcgtgccc ctctctagag ataatgagca ttgcatgtct
      121 aagttataaa aaattaccac atattttttt tgtcacactt gtttgaagtg cagtttatct
      181 atctttatac atatatttaa actttactct acgaataata taatctatag tactacaata
      241 atatcagtgt tttagagaat catataaatg aacagttaga catggtctaa aggacaattg
      301 agtattttga caacaggact ctacagtttt atctttttag tgtgcatgtg ttctcctttt
      361 tttttgcaaa tagcttcacc tatataatac ttcatccatt ttattagtac atccatttag
      421 ggtttagggt taatggtttt tatagactaa tttttttagt acatctattt tattctattt
      481 tagcctctaa attaagaaaa ctaaaactct attttagttt ttttatttaa taatttagat
      541 ataaaataga ataaaataaa gtgactaaaa attaaacaaa taccctttaa gaaattaaaa
      601 aaactaagga aacatttttc ttgtttcgag tagataatgc cagcctgtta aacgccgtcg
      661 atcgacgagt ctaacggaca ccaaccagcg aaccagcagc gtcgcgtcgg gccaagcgaa
      721 gcagacggca cggcatctct gtcgctgcct ctggacccct ctcgagagtt ccgctccacc
      781 gttggacttg ctccgctgtc ggcatccaga aattgcgtgg cggagcggca gacgtgagcc
      841 ggcacggcag gcggcctcct cctcctctca cggcaccggc agctacgggg gattcctttc
      901 ccaccgctcc ttcgctttcc cttcctcgcc cgccgtaata aatagacacc ccctccacac
      961 cctctttccc caacctcgtg ttgttcggag cgcacacaca cacaaccaga tctcccccaa
     1021 atccacccgt cggcacctcc gcttcaaggt acgccgctcg tcctcccccc ccccccctct
     1081 ctaccttctc tagatcggcg ttccggtcca tggttagggc ccggtagttc tacttctgtt
     1141 catgtttgtg ttagatccgt gtttgtgtta gatccgtgct gctagcgttc gtacacggat
     1201 gcgacctgta cgtcagacac gttctgattg ctaacttgcc agtgtttctc tttggggaat
     1261 cctgggatgg ctctagccgt tccgcagacg ggatcgatct aggataggta tacatgttga
     1321 tgtgggtttt actgatgcat atacatgatg gcatatgcag catctattca tatgctctaa
     1381 ccttgagtac ctatctatta taataaacaa gtatgtttta taattatttt gatcttgata
     1441 tacttggatg atggcatatg cagcagctat atgtggattt ttttagccct gccttcatac
     1501 gctatttatt tgcttggtac tgtttctttt gtcgatgctc accctgttgt ttggtgttac
     1561 ttctgcaggt actagttgga tcctgtagaa accccaaccc gtgaaatcaa aaaactcgac
     1621 ggcctgtggg cattcagtct ggatcgcgaa aactgtggaa ttgatcagcg ttggtgggaa
     1681 agcgcgttac aagaaagccg ggcaattgct gtgccaggca gttttaacga tcagttcgcc
     1741 gatgcagata ttcgtaatta tgcgggcaac gtctggtatc agcgcgaagt ctttataccg
     1801 aaaggttggg caggccagcg tatcgtgctg cgtttcgatg cggtcactca ttacggcaaa
     1861 gtgtgggtca ataatcagga agtgatggag catcagggcg gctatacgcc atttgaagcc
     1921 gatgtcacgc cgtatgttat tgccgggaaa agtgtacgta tcaccgtttg tgtgaacaac
     1981 gaactgaact ggcagactat cccgccggga atggtgatta ccgacgaaaa cggcaagaaa
     2041 aagcagtctt acttccatga tttctttaac tatgccggaa tccatcgcag cgtaatgctc
     2101 tacaccacgc cgaacacctg ggtggacgat atcaccgtgg tgacgcatgt cgcgcaagac
     2161 tgtaaccacg cgtctgttga ctggcaggtg gtggccaatg gtgatgtcag cgttgaactg
     2221 cgtgatgcgg atcaacaggt ggttgcaact ggacaaggca ctagcgggac tttgcaagtg
     2281 gtgaatccgc acctctggca accgggtgaa ggttatctct atgaactgtg cgtcacagcc
     2341 aaaagccaga cagaattcca agcttggcgc gccgagctca gtcgacgatc gttcaaacat
     2401 ttggcaataa agtttcttaa gattgaatcc tgttgccggt cttgcgatga ttatcatata
     2461 atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga cgttatttat
     2521 gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga tagaaaacaa
     2581 aatatagcgc gcaaactagg ataaattatc gcgcgcggtg tcatctatgt tactagatcg
     2641 gtacctataa cttcgtataa tgtatactat acgaagttat ttaaattggc ccccaaggcc
     2701 tctagtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     2761 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     2821 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     2881 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     2941 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     3001 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     3061 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     3121 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     3181 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     3241 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     3301 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     3361 aacgaaaact cacgttaagg gattttggcc atgagacaat aaccctgata aatgcttcaa
     3421 taatattgaa aaaggaagac catgattacc aatgcttaat cagtgaggca cctatctcag
     3481 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     3541 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     3601 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     3661 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     3721 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tgctggcatc gtggtgtcac
     3781 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     3841 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     3901 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     3961 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     4021 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcgc
     4081 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     4141 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     4201 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     4261 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatggct gtcagaccaa
     4321 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     4381 gtgaagatcc tttttgataa tctcatgggc ggatacatat ttgaatgtat ttagaaaaat
     4441 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cactagaggc cctta
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